A YAC contig containing the reeler locus with preliminary characterization of candidate gene fragments

Journal Article · Mon Apr 10 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1016/0888-7543(95)80173-J· OSTI ID:209937

Bar, I; Ruelle, D; Royaux, I ^[1]

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1. FUNDP School of Medicine, Namur (Belgium); and others

The reeler mutation in the mouse maps to proximal chromosome 5 and defines a key gene involved in brain development and evolution. No gene product is known, and the locus is currently being characterized by positional cloning. YAC clones corresponding to the closest markers D5Mit61 and D5Mit72 have been isolated. Cloned extremities of the YAC inserts were used to construct a 1.1-Mb contig, a 700-kb fragment of which was shown to contain the reeler locus. The integrity of the contig was verified by physical mapping on genomic DNA. The classical allele of the reeler mutation was associated with a 150-kb deletion between D5Mit61 and D5Mit72, while no gross chromosomal anomaly was found in the Orleans allele. Candidate coding sequences were isolated to construct a preliminary transcriptional map of the reeler region. Cosmid clones mapping within the rl deletion revealed a large transcript of more than 11 kb, which was present in normal embryonic brain but barely detectable in homozygous rl{sup Orl}/rl{sup Orl} embryonic brain, suggesting strongly that it corresponds to the reeler transcript. 30 refs., 5 figs., 3 tabs.

• OSTI ID: 209937
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 26; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMES
CONTIGS
DESIGN
DNA-CLONING
ELECTROPHORESIS
GENE MUTATIONS
GENES
GENETIC MAPPING
MICE
PROBES
TRANSCRIPTION
YEASTS