Atomic force microscopic investigation on the potential early intermediate stages of fibrillogenesis of fibronectin within fibrils

Journal Article · Fri Sep 21 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.07.014· OSTI ID:20991543

Yong, Chen ^[1]; Yangzhe, Wu ^[2]; Jiye, Cai ^[2]

---

1. Department of Chemistry, Jinan University, Guangzhou, Guangdong 510632 (China) and Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612 (United States)
2. Department of Chemistry, Jinan University, Guangzhou, Guangdong 510632 (China)

To date, a large number of literature have focused on the mechanisms of fibronectin (FN) fibril initiation and elongation, discovering many binding sites on FN molecules that are required for FN fibril growth. However, it is still poorly understood how FN fibrils widen while elongating. Here, single molecules and polymers, FN fibrillogenesis, and FN fibril bundles around cells have all been investigated visually using atomic force microscopy. We found that the formation of ring-shaped and beaded-filament-like FN aggregates may be two early intermediate stages of FN fibrillogenesis within the fibrils away from cells, perhaps involving in the FN fibril widening/bundling.

• OSTI ID: 20991543
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 361; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATOMIC FORCE MICROSCOPY
ELONGATION
FIBRIN
FILAMENTS
GROWTH
MATRICES
POLYMERS