Hyaluronan preserves the proliferation and differentiation potentials of long-term cultured murine adipose-derived stromal cells
- Department of Chemical Engineering, National Taiwan University, No.1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan (China)
- Institute of Biotechnology, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan (China) and Institute of Clinical Medicine, National Cheng Kung University, Tainan 70101, Taiwan (China)
For long-term culture, murine adipose-derived stromal cells (mADSCs) at latter passages demonstrated a marked decline in proliferative activity, exhibited senescent morphology and reduced differentiation potentials, particularly osteogenesis. To extend the lifespan of mADSCs, two culture conditions containing hyaluronan (HA) was compared in our study, one as a culture medium supplement (SHA), and the other where HA was pre-coated on culture surface (CHA). mADSCs cultivated with SHA exhibited a prolonged lifespan, reduced cellular senescence, and enhanced osteogenic potential compared to regular culture condition (control). Upon CHA treatment, mADSCs tended to form cell aggregates with gradual growth profiles, while their differentiation activities remained similar to SHA groups. After transferring mADSCs from CHA to control surface, they were shown to have an extended lifespan and an increase of osteogenic potential. Our results suggested that HA can be useful for preserving the proliferation and differentiation potentials of long-term cultured mADSCs.
- OSTI ID:
- 20991488
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 360, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2007.04.211; PII: S0006-291X(07)00861-3; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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