In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute

Journal Article · Fri Aug 18 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.06.072· OSTI ID:20854416

Abbah, S A ^[1]; Lu, W W ^[2]; Chan, D ^[1]; Cheung, K M.C. ^[1]; Liu, W G ^[3]; Zhao, F ^[3]; Li, Z Y ^[1]; Leong, J C.Y. ^[1]; Luk, K D.K. ^[1]

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1. Department of Orthopaedics and Traumatology, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong (China)
2. Department of Orthopaedics and Traumatology, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong (China) and Department of Biochemistry, University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong (China)
3. Research Institute of Polymeric Materials, Tianjin University, Tianjin (China)

This study aims to investigate the survival and osteogenic behavior of murine-derived adipose-tissue stromal cells (ATSCs) encapsulated in alginate microcapsules thereby instigating further studies in this cell delivery strategy for in vivo osteogenesis. Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis. Following microencapsulation, cell numbers increased from 3.9 x 10{sup 3} on day 1 to 7.8 x 10{sup 3} on day 7 and maintained excellent viability in the course of 21-day culture. ALP was 6.9, 5.5, and 3.2 times higher than monolayer cultures on days 7, 14, and 21, respectively. In addition, osteocalcin mRNA was detectable in encapsulated cultures earlier (day 14) than monolayer cultures. We conclude that alginate microcapsules can act as three-dimensional matrix for ATSC proliferation and has potential for use as injectable, biodegradable scaffold in bone tissue engineering.

• OSTI ID: 20854416
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 347, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2006.06.072; PII: S0006-291X(06)01366-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADIPOSE TISSUE
ALGINATES
ALKALINE PHOSPHATASE
BONE TISSUES
CELL PROLIFERATION
GRAFTS
IN VITRO
IN VIVO
SKELETON
TETRAZOLIUM