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Title: Identification of acid-sensing ion channels in adenoid cystic carcinomas

Abstract

Tissue acidosis is an important feature of tumor. The response of adenoid cystic carcinoma (ACC) cells to acidic solution was studied using whole-cell patch-clamp recording in the current study. An inward, amiloride-sensitive Na{sup +} current was identified in cultured ACC-2 cells while not in normal human salivary gland epithelial cells. Electrophysiological and pharmacological properties of the currents suggest that heteromeric acid-sensing ion channels (ASICs) containing 2a and 3 may be responsible for the proton-induced currents in the majority of ACC-2 cells. Consistent with it, analyses of RT-PCR and Western blotting demonstrated the presences of ASIC2a and 3 in ACC-2 cells. Furthermore, we observed the enhanced expression of ASIC2a and 3 in the sample of ACC tissues. These results indicate that the functional expression of ASICs is characteristic feature of ACC cells.

Authors:
 [1];  [2];  [3];  [2];  [4];  [1];  [5];  [6]
  1. Department of Oral and Maxillofacial Surgery, School of Stomatology, Affiliated Ninth People's Hospital, Shanghai Jiaotong University, Shanghai Research Institute of Stomatology, Shanghai 200011 (China)
  2. (China)
  3. Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)
  4. Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Research Institute of Stomatology, Nanjing 210029 (China)
  5. Department of Oral and Maxillofacial Surgery, School of Stomatology, Affiliated Ninth People's Hospital, Shanghai Jiaotong University, Shanghai Research Institute of Stomatology, Shanghai 200011 (China). E-mail: yjh98001@sjtu.edu.cn
  6. Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China). E-mail: tlxu@ion.ac.cn
Publication Date:
OSTI Identifier:
20979888
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 355; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.02.061; PII: S0006-291X(07)00342-7; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARCINOMAS; CYSTS; POLYMERASE CHAIN REACTION; SALIVARY GLANDS; SODIUM IONS

Citation Formats

Ye Jinhai, Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Research Institute of Stomatology, Nanjing 210029, Gao Jun, Model Animal Research Center and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210061, Wu Yunong, Hu Yongjie, Zhang Chenping, and Xu Tianle. Identification of acid-sensing ion channels in adenoid cystic carcinomas. United States: N. p., 2007. Web.
Ye Jinhai, Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Research Institute of Stomatology, Nanjing 210029, Gao Jun, Model Animal Research Center and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210061, Wu Yunong, Hu Yongjie, Zhang Chenping, & Xu Tianle. Identification of acid-sensing ion channels in adenoid cystic carcinomas. United States.
Ye Jinhai, Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Research Institute of Stomatology, Nanjing 210029, Gao Jun, Model Animal Research Center and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210061, Wu Yunong, Hu Yongjie, Zhang Chenping, and Xu Tianle. Fri . "Identification of acid-sensing ion channels in adenoid cystic carcinomas". United States. doi:.
@article{osti_20979888,
title = {Identification of acid-sensing ion channels in adenoid cystic carcinomas},
author = {Ye Jinhai and Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Research Institute of Stomatology, Nanjing 210029 and Gao Jun and Model Animal Research Center and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210061 and Wu Yunong and Hu Yongjie and Zhang Chenping and Xu Tianle},
abstractNote = {Tissue acidosis is an important feature of tumor. The response of adenoid cystic carcinoma (ACC) cells to acidic solution was studied using whole-cell patch-clamp recording in the current study. An inward, amiloride-sensitive Na{sup +} current was identified in cultured ACC-2 cells while not in normal human salivary gland epithelial cells. Electrophysiological and pharmacological properties of the currents suggest that heteromeric acid-sensing ion channels (ASICs) containing 2a and 3 may be responsible for the proton-induced currents in the majority of ACC-2 cells. Consistent with it, analyses of RT-PCR and Western blotting demonstrated the presences of ASIC2a and 3 in ACC-2 cells. Furthermore, we observed the enhanced expression of ASIC2a and 3 in the sample of ACC tissues. These results indicate that the functional expression of ASICs is characteristic feature of ACC cells.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 355,
place = {United States},
year = {Fri Apr 20 00:00:00 EDT 2007},
month = {Fri Apr 20 00:00:00 EDT 2007}
}