Analysis of the antimicrobial activities of a chemokine-derived peptide (CDAP-4) on Pseudomonas aeruginosa

Journal Article · Fri Apr 06 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.01.188· OSTI ID:20979868

Martinez-Becerra, Francisco ^[1]; Silva, Daniel-Adriano ^[2]; Dominguez-Ramirez, Lenin ^[2]; Mendoza-Hernandez, Guillermo ^[2]; Lopez-Vidal, Yolanda ^[3]; Soldevila, Gloria ^[1]; Garcia-Zepeda, Eduardo A ^[1]

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1. Department of Immunology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Circuito Escolar s/n, Mexico, DF, CP 04510 (Mexico)
2. Department of Biochemistry, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (Mexico)
3. Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (Mexico)

Chemokines are key molecules involved in the control of leukocyte trafficking. Recently, a novel function as antimicrobial proteins has been described. CCL13 is the only member of the MCP chemokine subfamily displaying antimicrobial activity. To determine Key residues involved in its antimicrobial activity, CCL13 derived peptides were synthesized and tested against several bacterial strains, including Pseudomonas aeruginosa. One of these peptides, corresponding to the C-terminal region of CCL13 (CDAP-4) displayed good antimicrobial activity. Electron microscopy studies revealed remarkable morphological changes after CDAP-4 treatment. By computer modeling, CDAP-4 in {alpha} helical configuration generated a positive electrostatic potential that extended beyond the surface of the molecule. This feature is similar to other antimicrobial peptides. Altogether, these findings indicate that the antimicrobial activity was displayed by CCL13 resides to some extent at the C-terminal region. Furthermore, CDAP-4 could be considered a good antimicrobial candidate with a potential use against pathogens including P. aeruginosa.

• OSTI ID: 20979868
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 355; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ELECTRON MICROSCOPY
HELICAL CONFIGURATION
LEUKOCYTES
MORPHOLOGICAL CHANGES
PATHOGENS
PEPTIDES
PSEUDOMONAS
SIMULATION