Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway

Journal Article · Fri Feb 09 04:00:00 EST 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.12.028· OSTI ID:20979786

Hayashi, Naoyuki ^[1]; kanazawa-u ac jp, E-mail: naoyuki@kenroku; Murakami, Seishi ^[2]; Tsurusaki, Susumu ^[3]; Nagaura, Zen-ichiro ^[3]; Oki, Masaya ^[3]; Nishitani, Hideo ^[3]; Kobayashi, Masahiko ^[1]; Shimizu, Hiroko ^[1]; Yamamoto, Ken-ichi ^[1]; Nishimoto, Takeharu ^[3]

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1. Department of Molecular Pathology, Cancer Research Institute, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-0934 (Japan)
2. Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-0934 (Japan)
3. Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582 (Japan)

RanGTPase is involved in many cellular processes. It functions in nuclear-cytosolic transport and centrosome formation. Ran also localizes to chromatin as RCC1 does, its guanine nucleotide exchange factor, but Ran's function on chromatin is not known. We found that gsp1, a temperature-sensitive mutant of GSP1, a Saccharomyces cerevisiae Ran homologue, suppressed the hydroxyurea (HU) and ultra violet (UV) sensitivities of the mec1 mutant. In UV-irradiated mec1 gsp1 cells, Rad53 was phosphorylated despite the lack of Mec1. This suppression depended on the TEL1 gene, given that the triple mutant, mec1 gsp1 tel1, was unable to grow. The gsp1 mutations also suppressed the HU sensitivity of the rad9 mutant in a Tel1-dependent manner, but not the HU sensitivity of the rad53 mutant. These results indicated that Rad53 was activated by the Tel1 pathway in mec1 gsp1 cells, suggesting that Gsp1 helps regulate the role switching the ATM family kinases Mec1 and Tel1.

• OSTI ID: 20979786
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 353; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CHROMATIN
GENES
GUANINE
HYDROXYUREA
INHIBITION
IRRADIATION
MUTANTS
MUTATIONS
NUCLEOTIDES
PHOSPHOTRANSFERASES
SACCHAROMYCES CEREVISIAE
SENSITIVITY