Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p

Journal Article · Fri Oct 21 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.08.108· OSTI ID:20713395

Yonggang, Wang ^[1]; Nakashima, Nobutaka ^[1]; Sekiguchi, Takeshi ^[1]; Nishimoto, Takeharu ^[1]

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1. Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812-8582 (Japan)

A Gtr1p GTPase, the GDP mutant of which suppresses both temperature-sensitive mutants of Saccharomyces cerevisiae RanGEF/Prp20p and RanGAP/Rna1p, was presently found to interact with Yrb2p, the S. cerevisiae homologue of mammalian Ran-binding protein 3. Gtr1p bound the Ran-binding domain of Yrb2p. In contrast, Gtr2p, a partner of Gtr1p, did not bind Yrb2p, although it bound Gtr1p. A triple mutant: yrb2{delta} gtr1{delta} gtr2{delta} was lethal, while a double mutant: gtr1{delta} gtr2{delta} survived well, indicating that Yrb2p protected cells from the killing effect of gtr1{delta} gtr2{delta}. Recombinant Gtr1p and Gtr2p were purified as a complex from Escherichia coli. The resulting Gtr1p-Gtr2p complex was comprised of an equal amount of Gtr1p and Gtr2p, which inhibited the Rna1p/Yrb2 dependent RanGAP activity. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p.

• OSTI ID: 20713395
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 336; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
ESCHERICHIA COLI
MUTANTS
PROTEINS
SACCHAROMYCES CEREVISIAE