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Title: Soluble metals in residual oil fly ash alter innate and adaptive pulmonary immune responses to bacterial infection in rats

Abstract

The soluble metals of the pollutant, residual oil fly ash (ROFA), have been shown to alter pulmonary bacterial clearance in rats. The goal of this study was to determine the potential effects on both the innate and adaptive lung immune responses after bacterial infection in rats pre-exposed to the soluble metals in ROFA. Sprague-Dawley rats were intratracheally dosed (i.t.) at day 0 with ROFA (R-Total) (1.0 mg/100 g body weight), the soluble fraction of ROFA (R-Soluble), the soluble sample subject to a chelator (R-Chelex), or phosphate-buffered saline (Saline). On day 3, rats were administered an i.t. dose of 5 x 10{sup 4} Listeria monocytogenes. On days 6, 8, and 10, bacterial pulmonary clearance was monitored and bronchoalveolar lavage (BAL) was performed on days 3 (pre-infection), 6, 8, and 10. A concentrated first fraction of lavage fluid was retained for analysis of lactate dehydrogenase and albumin to assess lung injury. BAL cell number, phenotype, and production of reactive oxygen (ROS) and nitrogen species (RNS) were assessed, and a variety of cytokines were measured in the BAL fluid. Rats pre-treated with R-Soluble showed elevated lung injury/cytotoxicity and increased cellular influx into the lungs. R-Soluble-treatment also altered ROS, RNS, and cytokine levels, andmore » caused a degree of macrophage and T cell inhibition. These effects of R-Soluble result in increased pulmonary bacterial burden after infection. The results suggest that soluble metals in ROFA increase lung injury and inflammation, and alter both innate and adaptive pulmonary immune responses.« less

Authors:
 [1];  [2];  [2];  [3];  [2];  [3]
  1. Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States) and West Virginia University, Morgantown, WV 26505 (United States). E-mail: jur6@cdc.gov
  2. Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States)
  3. (United States)
Publication Date:
OSTI Identifier:
20976959
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 221; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2007.03.022; PII: S0041-008X(07)00142-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALBUMINS; FLY ASH; INFLAMMATION; INJURIES; LACTATE DEHYDROGENASE; LAVAGE; LUNGS; LYMPHOKINES; MACROPHAGES; METALS; PETROLEUM RESIDUES; PHENOTYPE; PHOSPHATES; POLLUTANTS; RATS; TOXICITY

Citation Formats

Roberts, Jenny R., Young, Shih-Houng, Castranova, Vincent, West Virginia University, Morgantown, WV 26505, Antonini, James M., and West Virginia University, Morgantown, WV 26505. Soluble metals in residual oil fly ash alter innate and adaptive pulmonary immune responses to bacterial infection in rats. United States: N. p., 2007. Web. doi:10.1016/j.taap.2007.03.022.
Roberts, Jenny R., Young, Shih-Houng, Castranova, Vincent, West Virginia University, Morgantown, WV 26505, Antonini, James M., & West Virginia University, Morgantown, WV 26505. Soluble metals in residual oil fly ash alter innate and adaptive pulmonary immune responses to bacterial infection in rats. United States. doi:10.1016/j.taap.2007.03.022.
Roberts, Jenny R., Young, Shih-Houng, Castranova, Vincent, West Virginia University, Morgantown, WV 26505, Antonini, James M., and West Virginia University, Morgantown, WV 26505. Fri . "Soluble metals in residual oil fly ash alter innate and adaptive pulmonary immune responses to bacterial infection in rats". United States. doi:10.1016/j.taap.2007.03.022.
@article{osti_20976959,
title = {Soluble metals in residual oil fly ash alter innate and adaptive pulmonary immune responses to bacterial infection in rats},
author = {Roberts, Jenny R. and Young, Shih-Houng and Castranova, Vincent and West Virginia University, Morgantown, WV 26505 and Antonini, James M. and West Virginia University, Morgantown, WV 26505},
abstractNote = {The soluble metals of the pollutant, residual oil fly ash (ROFA), have been shown to alter pulmonary bacterial clearance in rats. The goal of this study was to determine the potential effects on both the innate and adaptive lung immune responses after bacterial infection in rats pre-exposed to the soluble metals in ROFA. Sprague-Dawley rats were intratracheally dosed (i.t.) at day 0 with ROFA (R-Total) (1.0 mg/100 g body weight), the soluble fraction of ROFA (R-Soluble), the soluble sample subject to a chelator (R-Chelex), or phosphate-buffered saline (Saline). On day 3, rats were administered an i.t. dose of 5 x 10{sup 4} Listeria monocytogenes. On days 6, 8, and 10, bacterial pulmonary clearance was monitored and bronchoalveolar lavage (BAL) was performed on days 3 (pre-infection), 6, 8, and 10. A concentrated first fraction of lavage fluid was retained for analysis of lactate dehydrogenase and albumin to assess lung injury. BAL cell number, phenotype, and production of reactive oxygen (ROS) and nitrogen species (RNS) were assessed, and a variety of cytokines were measured in the BAL fluid. Rats pre-treated with R-Soluble showed elevated lung injury/cytotoxicity and increased cellular influx into the lungs. R-Soluble-treatment also altered ROS, RNS, and cytokine levels, and caused a degree of macrophage and T cell inhibition. These effects of R-Soluble result in increased pulmonary bacterial burden after infection. The results suggest that soluble metals in ROFA increase lung injury and inflammation, and alter both innate and adaptive pulmonary immune responses.},
doi = {10.1016/j.taap.2007.03.022},
journal = {Toxicology and Applied Pharmacology},
number = 3,
volume = 221,
place = {United States},
year = {Fri Jun 15 00:00:00 EDT 2007},
month = {Fri Jun 15 00:00:00 EDT 2007}
}