The effect of trichlorfon and methylazoxymethanol on the development of guinea pig cerebellum

Journal Article · Thu Mar 15 04:00:00 EDT 2007 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2006.10.024· OSTI ID:20976877

Mehl, Anna ^[1]; Schanke, Tore M ^[1]; Torvik, Ansgar ^[2]; Fonnum, Frode ^[1]

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1. Norwegian Defense Research Establishment, Division for Protection and Material, N-2027, Kjeller (Norway)
2. Ulleval Hospital, Laboratory of Neuropathology, N-0407 Oslo (Norway)

The pesticide trichlorfon (125 mg/kg on days 42-44 in gestation) gives hypoplasia of Brain of the offspring without any significant reduction in their body weights. The hypoplasia may be caused by trichlorfon itself or by its metabolite dichlorvos. This period of development coincides with the growth spurt period of guinea pig brain. The largest changes occurred in the cerebellum. Electron microscopic examination of the cerebellar cortex showed increased apoptotic death of cells in the granule cell layer after trichlorfon treatment. A reduction in thickness of the external germinal layer of the cerebellar cortex and an elevated amount of pyknotic and karyorrhexic cells in the granule cell layer was found. There was a significant reduction in choline esterase, choline acetyltransferase and glutamate decarboxylase activities in the cerebellum. Methylazoxymethanol (15 mg/kg body weight, day 43) was examined for comparison and caused similar hypoplasia of the guinea pig cerebellum, but did also induce a reduction in body weight. Trichloroethanol, the main metabolite of trichlorfon, did not give brain hypoplasia.

• OSTI ID: 20976877
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 2-3 Vol. 219; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CEREBELLUM
CHOLINE
CHOLINESTERASE
DEATH
DECARBOXYLASES
GROWTH
GUINEA PIGS
PESTICIDES
PROGENY
THICKNESS