Analyzing the cellular contribution of bone marrow to fracture healing using bone marrow transplantation in mice

Journal Article · Fri Nov 24 04:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.09.079· OSTI ID:20854580

Colnot, C ^[1]; Huang, S ^[1]; Helms, J ^[2]

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1. University of California at San Francisco, Department of Orthopaedic Surgery, San Francisco General Hospital, San Francisco, CA 94110 (United States)
2. Stanford University, 257 Campus Drive, Stanford, CA 94306 (United States)

The bone marrow is believed to play important roles during fracture healing such as providing progenitor cells for inflammation, matrix remodeling, and cartilage and bone formation. Given the complex nature of bone repair, it remains difficult to distinguish the contributions of various cell types. Here we describe a mouse model based on bone marrow transplantation and genetic labeling to track cells originating from bone marrow during fracture healing. Following lethal irradiation and engraftment of bone marrow expressing the LacZ transgene constitutively, wild type mice underwent tibial fracture. Donor bone marrow-derived cells, which originated from the hematopoietic compartment, did not participate in the chondrogenic and osteogenic lineages during fracture healing. Instead, the donor bone marrow contributed to inflammatory and bone resorbing cells. This model can be exploited in the future to investigate the role of inflammation and matrix remodeling during bone repair, independent from osteogenesis and chondrogenesis.

• OSTI ID: 20854580
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 350; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL RADIATION EFFECTS
BONE FRACTURES
BONE MARROW
CARTILAGE
CONNECTIVE TISSUE CELLS
GALACTOSIDASE
HEALING
INFLAMMATION
LETHAL IRRADIATION
MICE
REPAIR
SKELETON