Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression

Journal Article · Fri Nov 10 04:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.08.188· OSTI ID:20854557

Xi, Li; Haiyan, Huang ^[1]; Jiegen, Chen; Lin, Jiang ^[1]; Honglei, Liu ^[1]; Deguo, Liu; Tanjing, Song ^[1]; Qun, He; Chungu, Ma ^[1]; Duan, Ma ^[1]; Houyan, Song ^[1]; Qiqun, Tang ^[1]

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1. Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University Shanghai Medical School, P.O. Box 238, 138 Yi Xue Yuan Road, Shanghai 200032 (China)

Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP){alpha} and peroxisome proliferator-activated receptor (PPAR) {gamma}, C/EBP{alpha}, and PPAR{gamma} turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBP{beta}, a transcriptional activator of the C/EBP{alpha} and PPAR{gamma} genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBP{alpha} and PPAR{gamma} genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBP{beta} occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G{sub 1}-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBP{beta}, and subsequently inhibited MCE as well as adipocyte differentiation.

• OSTI ID: 20854557
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 350; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DNA
GENES
INDUCTION
PHENOTYPE
RECEPTORS
TRANSCRIPTION