Bisindoylmaleimide I suppresses adipocyte differentiation through stabilization of intracellular {beta}-catenin protein
Journal Article
·
· Biochemical and Biophysical Research Communications
- PharmacoGenomics Research Center, Inje University, Busan 614-735 (Korea, Republic of)
- Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701 (Korea, Republic of)
- Department of Biochemistry, College of Medicine, Inje University, Busan 614-735 (Korea, Republic of)
The Wnt/{beta}-catenin signaling pathway plays important roles in cell differentiation. Activation of this pathway, likely by Wnt-10b, has been shown to inhibit adipogenesis in cultured 3T3-L1 preadipocytes and mice. Here we revealed that bisindoylmaleimide I (BIM), which is widely used as a specific inhibitor of protein kinase C (PKC), inhibits adipocyte differentiation through activation of the Wnt/{beta}-catenin signaling pathway. BIM increased {beta}-catenin responsive transcription (CRT) and up-regulated intracellular {beta}-catenin levels in HEK293 cells and 3T3-L1 preadipocytes. BIM significantly decreased intracellular lipid accumulation and reduced expression of important adipocyte marker genes including peroxisome-proliferator-activated receptor {gamma} (PPAR{gamma}) and CAATT enhancer-binding protein {alpha} (C/EBP{alpha}) in 3T3-L1 preadipocytes. Taken together, our findings indicate that BIM inhibits adipogenesis by increasing the stability of {beta}-catenin protein in 3T3-L1 preadipocyte cells.
- OSTI ID:
- 21043640
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 367; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
Similar Records
{beta}-Catenin mediates the anti-adipogenic effect of baicalin
Fucoxanthin exerts differing effects on 3T3-L1 cells according to differentiation stage and inhibits glucose uptake in mature adipocytes
Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3
Journal Article
·
Fri Aug 06 00:00:00 EDT 2010
· Biochemical and Biophysical Research Communications
·
OSTI ID:22202726
Fucoxanthin exerts differing effects on 3T3-L1 cells according to differentiation stage and inhibits glucose uptake in mature adipocytes
Journal Article
·
Fri Jun 17 00:00:00 EDT 2011
· Biochemical and Biophysical Research Communications
·
OSTI ID:22204966
Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3
Journal Article
·
Fri May 20 00:00:00 EDT 2016
· Biochemical and Biophysical Research Communications
·
OSTI ID:22598739