In vivo two-photon microscopy study of short-term effects of microbeam irradiation on normal mouse brain microvasculature

Journal Article · Sat Apr 01 04:00:00 EST 2006 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/J.IJROBP.2005.1· OSTI ID:20793439

Serduc, Raphael ^[1]; Verant, Pascale ^[2]; Vial, Jean-Claude ^[2]; Farion, Regine ^[1]; Rocas, Linda ^[1]; Remy, Chantal ^[1]; Fadlallah, Taoufik M.S. ^[1]; Brauer, Elke M.S. ^[3]; Bravin, Alberto ^[3]; Laissue, Jean ^[4]; Blattmann, Hans ^[5]; Sanden, Boudewijn van der ^[1]

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1. INSERM, U 594, Functional and Metabolic Neuroimaging, Grenoble (France) and Universite Joseph Fourier Grenoble, Grenoble (France)
2. CNRS, UMR 5588, Grenoble (France)
3. European Synchrotron Radiation Facility, Grenoble (France)
4. Institute of Pathology, University of Bern, Bern (Switzerland)
5. Paul Scherrer Institute, Villigen (Switzerland)

Purpose: The purpose of this study was to assess the early effects of microbeam irradiation on the vascular permeability and volume in the parietal cortex of normal nude mice using two-photon microscopy and immunohistochemistry. Methods and Materials: The upper part of the left hemisphere of 55 mice was irradiated anteroposteriorly using 18 vertically oriented beams (width 25 {mu}m, interdistance 211 {mu}m; peak entrance doses: 312 or 1000 Gy). At different times after microbeam exposure, the microvasculature in the cortex was analyzed using intravital two-photon microscopy after intravascular injection of fluorescein isothiocyanate (FITC)-dextrans and sulforhodamine B (SRB). Changes of the vascular volume were observed at the FITC wavelength over a maximum depth of 650 {mu}m from the dura. The vascular permeability was detected as extravasations of SRB. Results: For all times (12 h to 1 month) after microbeam irradiation and for both doses, the FITC-dextran remained in the vessels. No significant change in vascular volume was observed between 12 h and 3 months after irradiation. Diffusion of SRB was observed in microbeam irradiated regions from 12 h until 12 days only after a 1000 Gy exposure. Conclusion: No radiation damage to the microvasculature was detected in normal brain tissue after a 312 Gy microbeam irradiation. This dose would be more appropriate than 1000 Gy for the treatment of brain tumors using crossfired microbeams.

• OSTI ID: 20793439
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Journal Name: International Journal of Radiation Oncology, Biology and Physics Journal Issue: 5 Vol. 64; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD-BRAIN BARRIER
BRAIN
DEXTRAN
FLUORESCEIN
IN VIVO
INJECTION
IRRADIATION
ISOTHIOCYANATES
MICE
MICROSCOPY
NEOPLASMS
PERMEABILITY
PHOTONS
RADIATION DOSES
RADIOTHERAPY
SYNCHROTRON RADIATION
X RADIATION