Dominant negative selection of vaccinia virus using a thymidine kinase/thymidylate kinase fusion gene and the prodrug azidothymidine
- Baxter BioScience/Vaccines, Biomedical Research Center, Uferstrasse 15, A-2304 Orth/Donau (Austria)
The Escherichia coli thymidine kinase/thymidylate kinase (tk/tmk) fusion gene encodes an enzyme that efficiently converts the prodrug 3'-azido-2',3'-dideoxythymidine (AZT) into its toxic triphosphate derivative, a substance which stops DNA chain elongation. Integration of this marker gene into vaccinia virus that normally is not inhibited by AZT allowed the establishment of a powerful selection procedure for recombinant viruses. In contrast to the conventional vaccinia thymidine kinase (tk) selection that is performed in tk-negative cell lines, AZT selection can be performed in normal (tk-positive) cell lines. The technique is especially useful for the generation of replication-deficient vaccinia viruses and may also be used for gene knock-out studies of essential vaccinia genes.
- OSTI ID:
- 20726038
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 2 Vol. 337; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
Control of thymidine kinase synthesis in IHD vaccinia virus-infected thymidine kinase-deficient LM cells
Identification and nucleotide sequence of the thymidine kinase gene of Shope fibroma virus
Thymidine plaque autoradiography of thymidine kinase-positive and thymidine kinase-negative herpesviruses
Journal Article
·
Tue Jul 01 00:00:00 EDT 1975
· J. Virol., v. 16, no. 1, pp. 210-213
·
OSTI ID:4169836
Identification and nucleotide sequence of the thymidine kinase gene of Shope fibroma virus
Journal Article
·
Sun Nov 30 23:00:00 EST 1986
· J. Virol.; (United States)
·
OSTI ID:5982760
Thymidine plaque autoradiography of thymidine kinase-positive and thymidine kinase-negative herpesviruses
Journal Article
·
Fri Dec 31 23:00:00 EST 1982
· J. Clin. Microbiol.; (United States)
·
OSTI ID:6257026