Pharmacokinetic and pharmacodynamic interactions of morin and cyclosporin

Journal Article · Sun May 15 04:00:00 EDT 2005 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2004.09.006· OSTI ID:20721806

Fang, S -H ^[1]; Hou, Y -C ^[2]; Chao, P -D.L. ^[3]

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1. Department of Microbiology, School of Medicine, China Medical University, Taichung, Taiwan (China)
2. Department of Microbiology, School of Chinese Medicine, China Medical University, Taichung, Taiwan (China)
3. Department of Microbiology, School of Pharmacy, China Medical University, Taichung, Taiwan (China)

Morin is a flavonoid present in mulberry and herbs. We have reported that morin exerted anti-inflammatory activity on the activated macrophages. Cyclosporin (CsA) is a potent immunosuppressive agent with narrow therapeutic range, which is widely used for the treatments of autoimmune diseases and transplantation rejection. This study aimed to measure the effects of morin on the disposition of CsA in lymphoid and non-lymphoid tissues, and on the functions of immune cells in mice. CsA (Neoral, 10 mg/kg) was orally administered with and without a concomitant dose of morin (0, 50, 100, 200 mg/kg) to mice once daily for 2 weeks. CsA concentrations in blood, liver, kidney, and spleen were determined by a specific monoclonal fluorescence polarization immunoassay. The decreased levels of CsA in tissues were found well correlated to increased doses of morin. The coadministration of 200 mg/kg morin significantly decreased CsA in blood, liver, kidney, and spleen by 33%, 17%, 38%, and 45%, respectively. On the other hand, coadministration of morin decreased dramatically the nitric oxide production by the activated macrophages when compared to CsA treatment alone. Moreover, morin maintained the level of CsA-suppressed T helper 1 (Th1) type cytokine, although the CsA concentration in spleen was markedly reduced. In conclusion, morin coadministration profoundly reduced CsA concentration but did not significantly alter the CsA-suppressed Th1 immune response in mice.

• OSTI ID: 20721806
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 1 Vol. 205; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BLOOD
FLUORESCENCE
GLYCOPROTEINS
HERBS
IMMUNOASSAY
INFLAMMATION
KIDNEYS
LIVER
LYMPHOKINES
MACROPHAGES
MICE
MORIN
NITRIC OXIDE
SPLEEN