Depletion of FKBP does not affect the interaction between isolated ryanodine receptors

Journal Article · Fri Oct 14 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.08.051· OSTI ID:20711018

Xiaofang, Hu ^[1]; Xin, Liang ^[1]; Keying, Chen ^[1]; Peihong, Zhu ^[2]; Jun, Hu ^[1]; edu cn, E-mail: jhu@sjtu

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1. Bio-X Life Science Research Center, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 1954 Hua-Shan Road, Shanghai 200030 (China)
2. Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031 (China)

The ryanodine receptors/calcium release channels (RyRs) usually form two dimensional regular lattices in the endoplasmic/sarcoplasmic reticulum membranes. The native RyR is associated with many auxiliary proteins, including FKBP. It has been indicated that FKBP may play a role in the intermolecular interaction and coupled gating of neighboring RyRs. However, a more recent study shows that FKBP12 is not involved in the physical linkage between neighboring RyR1s. In the present work, the effect of FKBP12 on the interaction between RyR1s isolated from rabbit skeletal muscle was investigated in an aqueous medium with photon correlation spectroscopy. We found that the depletion of FKBP12 did not affect the oligomerization of RyR1s in the medium containing different [KCl] or under different channel functional states. No evidence is obtained for the involvement of FKBP12 in the intermolecular interaction between RyR1s.

• OSTI ID: 20711018
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 336; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CALCIUM
MEMBRANES
MODULATION
PHOTONS
POTASSIUM CHLORIDES
RABBITS
RECEPTORS
SARCOPLASMIC RETICULUM
SPECTROSCOPY