Target size of the ryanodine receptor from junctional terminal cisternae of sarcoplasmic reticulum

Journal Article · Tue Jun 02 00:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00385a036· OSTI ID:6019939

McGrew, S G; Boucek, Jr, R J; McIntyre, J O; Jung, C Y; Fleischer, S

Target inactivation analysis was carried out on the ryanodine receptor. This receptor recently has been implicated as the channel involved in the calcium release process in excitation-contraction coupling and was localized to the junctional terminal cisternae of sarcoplasmic reticulum from skeletal muscle. Irradiation of the junctional terminal cisternae resulted in an exponential decrease in ryanodine binding with radiation dose, thereby consistent with target theory. The target molecular weight was found to be 138,000 +/- 21,000, i.e., smaller than the polypeptide that binds ryanodine. The calcium pump protein in the same membrane preparation served as an internal control to validate the methodology.

• Research Organization: Vanderbilt Univ., Nashville, TN
• OSTI ID: 6019939
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:11; ISSN BICHA
• Country of Publication: United States
• Language: English

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560120* -- Radiation Effects on Biochemicals
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METALS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CALCIUM
CELL CONSTITUENTS
CELL MEMBRANES
CHOLINE
DRUGS
ELEMENTS
HYDROXY COMPOUNDS
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LIPOTROPIC FACTORS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METALS
MOLECULAR WEIGHT
MUSCLES
ORGANIC COMPOUNDS
ORGANOIDS
PROTEINS
QUATERNARY COMPOUNDS
RABBITS
RADIATION EFFECTS
REACTION KINETICS
RECEPTORS
SARCOPLASMIC RETICULUM
VERTEBRATES