Muscarinic receptors are involved in LMM3 tumor cells proliferation and angiogenesis

Journal Article · Fri Sep 09 00:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.07.031· OSTI ID:20710963

Rimmaudo, Laura Elizabeth ^[1]; Torre, Eulalia de la ^[1]; Sacerdote de Lustig, Eugenia ^[1]; Sales, Maria Elena ^[1]

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1. Area Investigacion, Instituto de Oncologia A.H. Roffo, Facultad de Medicina, Universidad de Buenos Aires, Av. San Martin 5481 CP 1417, Buenos Aires (Argentina)

Angiogenesis is a process of new blood vessel development from pre-existing vasculature and it plays an essential role in tumor growth and metastases. Here, we investigate the expression of muscarinic acetylcholine receptors (mAchR) and their participation in tumor cell proliferation and angiogenesis ability. Saturation binding assays with the tritiated muscarinic antagonist quinuclidinyl benzilate indicate that LMM3 cells derived from a murine mammary adenocarcinoma express a single class of functional mAchR. Competition binding assays with selective muscarinic antagonists indicate a predominance of M{sub 3} receptor subtype. The muscarinic agonist carbachol (CARB) stimulates LMM3 cell proliferation in a concentration dependent manner. The maximal effect induced by 10{sup -9} M CARB was totally blunted by atropine and by the selective M{sub 3} and M{sub 1} antagonists, para-fluoro hexahydro sila-difenidol (pf-HHSiD) and pirenzepine, respectively. In addition, pf-HHSiD completely blocked in vivo CARB-induced neovascular formation and vascular endothelial growth factor-A in LMM3 tumor cells. We can conclude that mAchR expressed in LMM3 mammary tumor cells positively regulate proliferation and angiogenesis required for tumor progression.

• OSTI ID: 20710963
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 334, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2005.07.031; PII: S0006-291X(05)01443-9; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACETYLCHOLINE
ATROPINE
BLOOD VESSELS
CARCINOMAS
CELL PROLIFERATION
GROWTH
GROWTH FACTORS
IN VIVO
METASTASES
RECEPTORS
TUMOR CELLS