Nucleolin is regulated both at the level of transcription and translation

Journal Article · Fri Jul 08 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.05.022· OSTI ID:20710846

Bicknell, Katrina ^[1]; Brooks, Gavin ^[1]; Kaiser, Pete ^[2]; Hongying, Chen ^[3]; Dove, Brian K ^[4]; Hiscox, Julian A ^[4]

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1. Cardiovascular Research Group, School of Pharmacy, University of Reading (United Kingdom)
2. Institute for Animal Health, Compton (United Kingdom)
3. Virology Group, School of Animal and Microbial Sciences, University of Reading (United Kingdom)
4. School of Biochemistry and Microbiology, University of Leeds (United Kingdom)

Nucleolin is a multi-functional protein that is located to the nucleolus. In tissue culture cells, the stability of nucleolin is related to the proliferation status of the cell. During development, rat cardiomyocytes proliferate actively with increases in the mass of the heart being due to both hyperplasia and hypertrophy. The timing of this shift in the phenotype of the myocyte from one capable of undergoing hyperplasia to one that can grow only by hypertrophy occurs within 4 days of post-natal development. Thus, cardiomyocytes are an ideal model system in which to study the regulation of nucleolin during growth in vivo. Using Western blot and quantitative RT-PCR (TaqMan) we found that the amount of nucleolin is regulated both at the level of transcription and translation during the development of the cardiomyocyte. However, in cells which had exited the cell cycle and were subsequently given a hypertrophic stimulus, nucleolin was regulated post-transcriptionally.

• OSTI ID: 20710846
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 332; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL CELLS
CELL CYCLE
GENE REGULATION
GROWTH
HEART
HYPERTROPHY
IN VIVO
PHENOTYPE
POLYMERASE CHAIN REACTION
PROTEINS
RATS
TISSUE CULTURES
TRANSCRIPTION