Development of a protein microarray using sequence-specific DNA binding domain on DNA chip surface

Journal Article · Fri Apr 22 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.01.167· OSTI ID:20709153

Choi, Yoo Seong ^[1]; Pack, Seung Pil ^[2]; Yoo, Young Je ^[1]

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1. School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 (Korea, Republic of)
2. International Innovation Center, Kyoto University, Kyoto 606-8501 (Japan)

A protein microarray based on DNA microarray platform was developed to identify protein-protein interactions in vitro. The conventional DNA chip surface by 156-bp PCR product was prepared for a substrate of protein microarray. High-affinity sequence-specific DNA binding domain, GAL4 DNA binding domain, was introduced to the protein microarray as fusion partner of a target model protein, enhanced green fluorescent protein. The target protein was oriented immobilized directly on the DNA chip surface. Finally, monoclonal antibody of the target protein was used to identify the immobilized protein on the surface. This study shows that the conventional DNA chip can be used to make a protein microarray directly, and this novel protein microarray can be applicable as a tool for identifying protein-protein interactions.

• OSTI ID: 20709153
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 329; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AFFINITY
BORON PHOSPHIDES
DNA
IN VITRO
MONOCLONAL ANTIBODIES
POLYMERASE CHAIN REACTION
PROTEINS
SUBSTRATES
SURFACES