A 100 % Duty-ratio 3 x 6 Arrayed CCD X-ray Detector for Rapid Data Collection in Macromolecular Crystallography

Journal Article · Wed May 12 00:00:00 EDT 2004 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.1757944· OSTI ID:20653103

Ito, Kazuki ^[1]; Gaponov, Yurii ^[2]; Sakabe, Noriyoshi ^[3]; Amemiya, Yoshiyuki ^[4]

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1. Institute of Materials Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8573 Ibaraki (Japan)
2. Photon Factory, Institute of Materials Structure Science, KEK, 1-1 Oho, Tsukuba, 305-0801 Ibaraki (Japan)
3. Foundation for Advancement of International Science (FAIS), PF/IMSS KEK, Tsukuba, 305-0801 Ibaraki (Japan)
4. Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, 277-8561 Chiba (Japan)

A 3 x 6 arrayed charge coupled device (CCD) X-ray detector has been developed for rapid data collection in macromolecular crystallography at Photon Factory. The total active area is 236 mm x 236 mm (76.8 {mu}m x 76.8 {mu}m / pixel at the input phosphor, 3072 x 3072 pixels). Each module consists of an input phosphor, a 3.2 : 1 fiber- optic-taper (FOT), a CCD, and a readout circuit. The input phosphor is Gd2O2S:Tb (P-43), which is deposited onto an aluminum coated Mylar sheet to increase the sensitivity. The outputs from eighteen (3 x 6) CCDs are readout in parallel and then are digitized by 16 bit analog-to-digital converters. The advantage of this detector over the conventional FOT-coupled CCD detectors is a unique CCD readout scheme that enables us to record successive x-ray exposures without interruption among them. A shutter is not needed for CCD readout and the sample crystal can be rotated continuously without interruption during data acquisition. We have collected one data set of a Lysozyme crystal for 180 degree with 1.5 degree per slice in 360 seconds. The exposure time of each slice is 3 seconds. The duty-ratio of the x-ray exposure to the data collection time is 100 %. This detector combined with a synchrotron radiation source is very suitable for rapid data collection in macromolecular crystallography.

• OSTI ID: 20653103
• Journal Information: AIP Conference Proceedings, Vol. 705, Issue 1; Conference: 8. international conference on synchrotron radiation instrumentation, San Francisco, CA (United States), 25-29 Aug 2003; Other Information: DOI: 10.1063/1.1757944; (c) 2004 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
ALUMINIUM
ANALOG-TO-DIGITAL CONVERTERS
CHARGE-COUPLED DEVICES
CRYSTAL STRUCTURE
CRYSTALLOGRAPHY
CRYSTALS
DATA ACQUISITION
DATA ACQUISITION SYSTEMS
FIBER OPTICS
LYSOZYME
MYLAR
PHOTONS
READOUT SYSTEMS
SENSITIVITY
SYNCHROTRON RADIATION SOURCES
X RADIATION
X-RAY DETECTION