Single Cell Irradiation Nuclear Microscopy Using a Radioactive Source
- Sandia National Laboratories, Albuquerque, NM (United States)
- Laboratori Nazionali Legnaro, INFN, Legnaro, PD (Italy)
- Azienda Ospedaliera, Padova (Italy)
- Italy
- Quantar Technology inc., Santa Clara, CA (United States)
- Department of Experimental Physics of the University, 10125 Turin (Italy)
Irradiation of a single biological cell, instead of a whole tissue, with ions in a known number and position, is a powerful means to study very low dose biological effectiveness. Present methods employ accelerated ion beams which are 1) either collimated with micro-apertures and affected by a halo of 3-5{mu}m at best, or 2) focused to a sub-micron spot, whose resolution is degraded when extracted into air. We have studied the efficacy of a new micro-radiobiological method, originally developed for materials research. This new approach uses an IPEM, Ion Photon Emission Microscope, which employs a specially shaped Po-210 alpha particle source for in-air irradiation. Alpha particles strike the cells, which are previously grown directly on a 10-20 {mu}m thick scintillating plastic blade and placed in the focal plane of a conventional optical microscope. Photons produced at the single ion impact point are projected at high magnification onto a single-photon position sensitive detector, which provides the position of each ion that hits the cells. Adequacy of this setup for Single Cell Radio-Biology will be discussed.
- OSTI ID:
- 20632599
- Journal Information:
- AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 680; ISSN APCPCS; ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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