Uptake, metabolism and macromolecular binding of benzo(a)pyrene (BaP) metabolites in channel catfish
Conference
·
OSTI ID:203633
- State Univ. of New York, Stony Brook, NY (United States). Marine Sciences Research Center
Polynuclear aromatic hydrocarbons (PAH), such as BaP, are contaminants with demonstrated carcinogenicity, yet little is known about the fate of PAH metabolites in aquatic organisms. Some invertebrates actively metabolize BaP to hydroxylated and conjugated forms, becoming BaP metabolite-rich prey items for fish. The authors examined the fate of BaP metabolites acquired by fish through diet. Channel catfish, Ictaluruspunctatus, were fed a single dose (2.5 umoles/kg fish) of either {sup 3}H-parent BaP or individual {sup 3}H-BaP metabolites: 3-hydroxy, 9-hydroxy, 9-sulfate or 9-glucuronidated BaP. After 24 hrs fish were killed, and samples of intestinal mucosa, bile, liver and muscle immediately frozen at {minus}80 C. Mean (n = 3--5) percent of dose retained was lowest for conjugated BaP (3.7%), 2--4 times higher for hydroxylated BaP (8.9%), and 5 times higher for parent BaP (18.9%). Differences were also observed between the distributions of individual metabolites in tissues. Radioactivity derived from parent and hydroxylated BaP was several fold higher in intestinal mucosa and bile than in liver or muscle. In contrast, radioactivity derived from conjugated BaP metabolites was more evenly distributed between all four tissues. These data demonstrate that in fish (1) dietary BaP metabolites are absorbed by the intestine, and they or their metabolites are transported to and absorbed by major tissue reservoirs (liver, muscle), (2) conjugated metabolites are less readily absorbed and/or retained than parent or hydroxylated BaP, and (3) some differences in metabolic fate exist between metabolites from the same class. DNA, RNA and protein adducts, as well as metabolite profiles, are being determined in liver and intestine.
- OSTI ID:
- 203633
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
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