Rapid and accurate determination of Stern-Volmer quenching constants

Journal Article · Sun Aug 01 04:00:00 EDT 1999 · Applied Spectroscopy

DOI:https://doi.org/10.1366/0003702991947676· OSTI ID:20215279

Goodpaster, John V ^[1]; McGuffin, Victoria L ^[1]

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1. Department of Chemistry, Michigan State University, East Lansing, Michigan 48824-1322 (United States)

In this work, a novel system has been designed, characterized, and validated for the determination of fluorescence quenching constants. Capillary flow injection methods are used to automate the preparation and mixing of the fluorophore and quencher solutions. Because of the small diameter of the capillary (75-200 {mu}m), fluorescence measurements can be made without corrections for primary and secondary absorbance effects. The fluorescence spectrometer is equipped with a charge-coupled device (CCD) that has a detection limit of 3.0x10{sup -9} M (2.3 ppb) and a linear dynamic range of 10{sup 5} for integration times of 0.01-10 s. This spectrometer has a 300 nm spectral range with 1 nm resolution, allowing the fluorescence quenching constants to be calculated at single wavelengths or over integrated wavelength ranges. This system was validated by comparison to traditional methods for the determination of Stern-Volmer constants for alternant and nonalternant polycyclic aromatic hydrocarbons with nitromethane and triethylamine. (c) 2000 Society for Applied Spectroscopy.

• OSTI ID: 20215279
• Journal Information: Applied Spectroscopy, Journal Name: Applied Spectroscopy Journal Issue: 8 Vol. 53; ISSN 0003-7028; ISSN APSPA4
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
AROMATICS
CHEMICAL COMPOSITION
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
HYDROCARBONS
ULTRAVIOLET SPECTRA
VISIBLE SPECTRA