Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria
Journal Article
·
· Applied and Environmental Microbiology
A fluorescence-labeled wheat germ agglutinin staining technique was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.
- Research Organization:
- Idaho National Lab. (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC07-99ID13727
- OSTI ID:
- 20075765
- Journal Information:
- Applied and Environmental Microbiology, Vol. 66, Issue 5; Other Information: PBD: May 2000; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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