Combined immunofluorescence-DNA-fluorescence staining technique for enumeration of Thiobacillus ferrooxidans in a population of acidophilic bacteria
An antiserum raised against whole cells of Thiobacillus ferroxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferroooxidans in the microbial desulfurization process. Leaching experiments were performed, and the numbers of T. ferrooxidans cells and other bacteria were estimated by using a combined immunofluorescence-DNA-fluorescence staining technique that was adapted for this purpose. Nonsterile coal samples inoculated with T. ferrooxidans yielded high concentrations of soluble iron after 16 days. After this period, however, T. ferrooxidans cells could no longer be detected by the immunofluorescence assay, whereas the DNA-fluorescence staining procedure demonstrated a large number of microorganisms on the coal particles. These results indicate that T. ferrooxidans is removed by competition with different acidophilic microorganisms that were originally present on the coal.
- Research Organization:
- Univ. of Leiden, Netherlands
- OSTI ID:
- 6559211
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 53:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COAL
DESULFURIZATION
PYRITE
METABOLISM
THIOBACILLUS FERROXIDANS
FLUORESCENCE
BACTERIA
LEACHING
REMOVAL
STAINS
BACILLUS
CARBONACEOUS MATERIALS
CHALCOGENIDES
CHEMICAL REACTIONS
DISSOLUTION
ENERGY SOURCES
FOSSIL FUELS
FUELS
IRON COMPOUNDS
IRON SULFIDES
LUMINESCENCE
MATERIALS
MICROORGANISMS
MINERALS
SEPARATION PROCESSES
SULFIDE MINERALS
SULFIDES
SULFUR COMPOUNDS
SULFUR-OXIDIZING BACTERIA
TRANSITION ELEMENT COMPOUNDS
010402* - Coal
Lignite
& Peat- Purification & Upgrading
560300 - Chemicals Metabolism & Toxicology