Assessment of intestinal absorption: A methodology based on stable isotope adminstration and proton activation analysis

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:199851

Cantone, M C ^[1]

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1. Univ. of Milano (Italy)

The interest in biokinetic studies is driven by problems related to the physiopathology of oligoelements, chemical elemental pollution and radioactive release in case of nuclear accidents. The application of stable isotopes as tracers in studies of trace elements in the area of nutritional and food science is particularly attractive and specifically if considering the investigations on the most radiosensitive age groups of the population and the repeated studies on healthy people for the assessment of the bioavailability of different compounds. A tracer method based on stable isotope administration, which combines the simultaneous use of two tracers and proton activation analysis is presented. A study aimed to obtain molybdenum biokinetic data in humans was performed. One tracer ({sup 96}Mo) was orally administered and another ({sup 95}Mo) was intravenously injected to two fasting volunteer subjects. Venous blood samples were withdrawn at different postinjection times. The concentration in plasma for both the isotopes was determined by measuring the intensities of the gamma-lines from the technetium radioisotopes produced via (p,n) reactions. In the adopted experimental conditions a minimum detectable concentration of 2 ng isotope/ml plasma was attained. The parameters describing molybdenum kinetics were obtained for the two individuals. Moreover, the investigation was repeated with different tracer amounts for one of the two subjects, in both fasting and non-fasting condition.

• OSTI ID: 199851
• Report Number(s): CONF-941129--
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL ACCUMULATION
INTESTINAL ABSORPTION
MOLYBDENUM
MOLYBDENUM 95
MOLYBDENUM 96
TRACER TECHNIQUES