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Discovery of a novel transcriptional regulator of sugar catabolism in archaea

Journal Article · · Molecular Microbiology
DOI:https://doi.org/10.1111/mmi.15114· OSTI ID:1987902
 [1];  [1];  [1];  [2];  [3];  [4];  [4];  [5];  [3];  [1]
  1. Institut für Allgemeine Mikrobiologie, Christian‐Albrechts‐Universität Kiel Kiel Germany
  2. Department of Biochemistry and Molecular Biology Medical University of South Carolina Charleston South Carolina USA
  3. Department of Biochemistry &, Molecular Biology University of South Alabama Mobile Alabama USA
  4. Department of Microbiology and Cell Science Institute of Food and Agricultural Science, University of Florida Gainesville Florida USA
  5. Department of Microbiology and Cell Science Institute of Food and Agricultural Science, University of Florida Gainesville Florida USA, Genetics Institute, University of Florida Gainesville Florida USA
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Abstract

The haloarchaeon Haloferax volcanii degrades D‐glucose via the semiphosphorylative Entner‐Doudoroff pathway and D‐fructose via a modified Embden‐Meyerhof pathway. Here, we report the identification of GfcR, a novel type of transcriptional regulator that functions as an activator of both D‐glucose and D‐fructose catabolism. We find that in the presence of D‐glucose, GfcR activates gluconate dehydratase, glyceraldehyde‐3‐phosphate dehydrogenase and pyruvate kinase and also acts as activator of the phosphotransferase system and of fructose‐1,6‐bisphosphate aldolase, which are involved in uptake and degradation of D‐fructose. In addition, glyceraldehyde‐3‐phosphate dehydrogenase and pyruvate kinase are activated by GfcR in the presence of D‐fructose and also during growth on D‐galactose and glycerol. Electrophoretic mobility shift assays indicate that GfcR binds directly to promoters of regulated genes. Specific intermediates of the degradation pathways of the three hexoses and of glycerol were identified as inducer molecules of GfcR. GfcR is composed of a phosphoribosyltransferase (PRT) domain with an N‐terminal helix‐turn‐helix motif and thus shows homology to PurR of Gram‐positive bacteria that is involved in the transcriptional regulation of nucleotide biosynthesis. We propose that GfcR of H. volcanii evolved from a PRT‐like enzyme to attain a function as a transcriptional regulator of central sugar catabolic pathways in archaea.

Research Organization:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Organization:
German Research Foundation (DFG); National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)
Grant/Contract Number:
FG02-05ER15650
OSTI ID:
1987902
Alternate ID(s):
OSTI ID: 2419046
OSTI ID: 1995299
Journal Information:
Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 2 Vol. 120; ISSN 0950-382X
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
FAO
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
D-glucose and D-fructose catabolism
EMSA
Haloferax volcanii
Microbiology
archaea
electrophoretic mobility shift assay
phosphoribosyltransferase (PRT) protein family
transcriptional regulation