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GlpR Is a Direct Transcriptional Repressor of Fructose Metabolic Genes in Haloferax volcanii

Journal Article · · Journal of Bacteriology
DOI:https://doi.org/10.1128/jb.00244-18· OSTI ID:1541706
 [1];  [2];  [3];  [2];  [4];  [2];  [2];  [4];  [2];  [5]
  1. Univ. of Florida, Gainesville, FL (United States); DOE/OSTI
  2. Univ. of Florida, Gainesville, FL (United States)
  3. Univ. of Florida, Gainesville, FL (United States); Washington State Univ., Richland, WA (United States)
  4. Duke Univ., Durham, NC (United States)
  5. Univ. of Illinois at Urbana Champaign, Urbana, IL (United States)
+ Show Author Affiliations
DeoR-type helix-turn-helix (HTH) domain proteins are transcriptional regulators of sugar and nucleoside metabolism in diverse bacteria and also occur in select archaea. In the model archaeon Haloferax volcanii, previous work implicated GlpR, a DeoR-type transcriptional regulator, in the transcriptional repression of glpR and the gene encoding the fructose-specific phosphofructokinase (pfkB) during growth on glycerol. However, the global regulon governed by GlpR remained unclear. Here, we compared transcriptomes of wild-type and ΔglpR mutant strains grown on glycerol and glucose to detect significant transcript level differences for nearly 50 new genes regulated by GlpR. By coupling computational prediction of GlpR binding sequences with in vivo and in vitro DNA binding experiments, we determined that GlpR directly controls genes encoding enzymes involved in fructose degradation, including fructose bisphosphate aldolase, a central control point in glycolysis. GlpR also directly controls other transcription factors. In contrast, other metabolic pathways appear to be under the indirect influence of GlpR. In vitro experiments demonstrated that GlpR purifies to function as a tetramer that binds the effector molecule fructose-1-phosphate (F1P). Furthermore, these results suggest that H. volcanii GlpR functions as a direct negative regulator of fructose degradation during growth on carbon sources other than fructose, such as glucose and glycerol, and that GlpR bears striking functional similarity to bacterial DeoR-type regulators.
Research Organization:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
FG02-05ER15650
OSTI ID:
1541706
Journal Information:
Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 17 Vol. 200; ISSN 0021-9193
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (3)

The TK0271 Protein Activates Transcription of Aromatic Amino Acid Biosynthesis Genes in the Hyperthermophilic Archaeon Thermococcus kodakarensis journal October 2019
Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen journal September 2019
Conserved principles of transcriptional networks controlling metabolic flexibility in archaea journal December 2018

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59 BASIC BIOLOGICAL SCIENCES
Archaea
catabolite repression
fructose metabolism
glucose metabolism
glycerol
microbiology
transcription factor