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Data-driven Mapping of the Mouse Connectome: The utility of transfer learning to improve the performance of deep learning models performing axon segmentation on light-sheet microscopy images

Technical Report ·
DOI:https://doi.org/10.2172/1985702· OSTI ID:1985702
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  1. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
  2. Oregon Health & Science Univ., Portland, OR (United States)

Light sheet microscopy has made possible the high temporal and spatial 3D imaging of both fixed and live biological tissue, with samples as large as the entire mouse brain. However, segmentation and quantification of that data remains a time-consuming manual process. Machine learning methods promise the possibility of automating this process. This study seeks to advance the performance of prior models through the application of refinements such as transfer learning.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE Laboratory Directed Research and Development (LDRD) Program
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1985702
Report Number(s):
PNNL--33353
Country of Publication:
United States
Language:
English