Scattered–light–sheet microscopy with sub–cellular resolving power

Subedi, Nava R.; Stolyar, Sergey; Tuson, Sabrina J.; Marx, Christopher J.; Vasdekis, Andreas E.

doi:10.1002/jbio.202300068

Scattered–light–sheet microscopy with sub–cellular resolving power

Journal Article · Wed Jun 07 00:00:00 EDT 2023 · Journal of Biophotonics

DOI:https://doi.org/10.1002/jbio.202300068· OSTI ID:1985268

Subedi, Nava R. ^[1]; Stolyar, Sergey ^[2]; Tuson, Sabrina J. ^[2]; Marx, Christopher J. ^[2]; ^[2]

University of Idaho, Moscow, ID (United States); Regents of the University of Idaho
University of Idaho, Moscow, ID (United States)

Since its first demonstration over 100 years ago, scattering-based light-sheet microscopy has recently re-emerged as a key modality in label-free tissue imaging and cellular morphometry; however, scattering-based light-sheet imaging with subcellular resolution remains an unmet target to date. This is because related approaches inevitably superimpose speckle or granular intensity modulation on to the native subcellular features. Here, we addressed this challenge by deploying a time-averaged pseudo-thermalized light-sheet illumination. While pseudo-thermalizing the illumination sheet increased its lateral dimensions, we achieved subcellular resolving power at ultralow irradiance levels after image deconvolution. We validated this approach by imaging cytosolic carbon depots in yeast and bacteria with increased specificity and no staining. Altogether, we expect this scattering-based light-sheet microscopy approach will advance live single-cell imaging investigations by conferring low-irradiance and label-free operation towards eradicating phototoxicity.

View Accepted Manuscript (DOE)

Research Organization:: University of Idaho, Moscow, ID (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0022318

OSTI ID:: 1985268

Alternate ID(s):: OSTI ID: 1985714

Journal Information:: Journal of Biophotonics, Journal Name: Journal of Biophotonics Journal Issue: 9 Vol. 16; ISSN 1864-063X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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