Integrative Quantitative-Phase and Airy Light-Sheet Imaging

Subedi, Nava R.; Jung, Pawel S.; Bredeweg, Erin L.; Nemati, Shahla H.; Baker, Scott E.; Christodoulides, Demetrios N.; Vasdekis, Andreas E.

doi:10.1038/s41598-020-76730-x

Integrative Quantitative-Phase and Airy Light-Sheet Imaging

Journal Article · Tue Dec 01 04:00:00 EST 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-76730-x· OSTI ID:1725826

Subedi, Nava R. ^[1]; Jung, Pawel S. ^[2]; ^[3]; Nemati, Shahla H. ^[1]; ^[3]; Christodoulides, Demetrios N. ^[2]; Vasdekis, Andreas E. ^[4]

University of Idaho
University of Central Florida
BATTELLE (PACIFIC NW LAB)
UNIVERSITY OF IDAHO

Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free organelle recognition and metabolic information that are inaccessible by conventional methods. We report the fusion of these two modalities onto a standard inverted microscope that retains compatibility with microfluidics. We describe the utilization of an accelerating Airy-beam light-sheet yielding identical imaging areas with interferometry, and an application in unmasking the effects of cellular noise on metabolic compartmentalization.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1725826

Report Number(s):: PNNL-SA-153507

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 10

Country of Publication:: United States

Language:: English

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