Integrative quantitative-phase and airy light-sheet imaging

Subedi, N. R.; Jung, P. S.; Bredeweg, E. L.; Nemati, S.; Baker, S. E.; Christodoulides, D. N.; Vasdekis, A. E.

doi:10.1038/s41598-020-76730-x

Integrative quantitative-phase and airy light-sheet imaging

Journal Article · Thu Nov 19 00:00:00 EST 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-76730-x· OSTI ID:1720187

Subedi, N. R.; Jung, P. S.; Bredeweg, E. L.; Nemati, S.; Baker, S. E.; Christodoulides, D. N.; Vasdekis, A. E.

Abstract

Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.

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Research Organization:: Univ. of Idaho, Moscow, ID (United States)

Sponsoring Organization:: Polish Ministry of Higher Education; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019249

OSTI ID:: 1720187

Alternate ID(s):: OSTI ID: 1724091
OSTI ID: 1725826

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 10; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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