A near-infrared optical nanosensor for measuring aerobic respiration in microbial systems

Saccomano, Samuel C.; Cash, Kevin J.

doi:10.1039/d1an01855h

A near-infrared optical nanosensor for measuring aerobic respiration in microbial systems

Journal Article · Wed Nov 24 23:00:00 EST 2021 · Analyst

DOI:https://doi.org/10.1039/d1an01855h· OSTI ID:1982184

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Colorado School of Mines, Golden, CO (United States)

We developed a ratiometric oxygen-sensitive nanosensor and demonstrated application in monitoring metabolic oxygen consumption in microbial samples over time. Based on a near-infrared (NIR) emitting oxygen-quenched luminophore, platinum(II) octaethylporphine ketone (PtOEPK), along with a stable dioctadecyl dicarbocyanine reference dye (DiD), this nanosensor system provides an advantageous approach for overcoming imaging issues in biological systems, such as autofluorescence and optical scattering in the visible wavelength region. The dyes are encapsulated within a polymer-based nanoparticle matrix to maintain them at a constant ratio in biological samples, precluding the need for complex synthetic approaches. With this constant ratio of the two dyes, the nanosensor response can be measured as a ratio of their two signals, accounting for nanosensor concentration artifacts in measurements. The nanosensors are reversible, which enabled us to temporally monitor systems in which dissolved oxygen concentrations both increase and decrease. These sensors were applied for the monitoring of oxygen in samples of Saccharomyces cerevisiae (brewing yeast) in a 96-well optical fluorescence plate reader format over 60 h. By mixing the nanosensors directly into the sample well with the yeast, we were able to dynamically track metabolic activity changes over time due to varying cell concentration and exposure to an antimicrobial agent. As a result, this system could be a potential platform for high-throughput screening of various species or variants of microbes with unknown metabolic rates in response to external stimuli (antimicrobials, metabolites, etc.).

View Accepted Manuscript (DOE)

Research Organization:: US Department of Energy (USDOE), Washington, DC (United States). Office of Science, Biological and Environmental Research (BER)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI ID:: 1982184

Alternate ID(s):: OSTI ID: 1833740

Journal Information:: Analyst, Journal Name: Analyst Journal Issue: 1 Vol. 147; ISSN 0003-2654

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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