Linking the Salmonella enterica 1,2-Propanediol Utilization Bacterial Microcompartment Shell to the Enzymatic Core via the Shell Protein PduB

Kennedy, Nolan W.; Mills, Carolyn E.; Abrahamson, Charlotte H.; Archer, Andre G.; Shirman, Sasha; Jewett, Michael C.; Mangan, Niall M.; Tullman-Ercek, Danielle

doi:10.1128/jb.00576-21

Linking the Salmonella enterica 1,2-Propanediol Utilization Bacterial Microcompartment Shell to the Enzymatic Core via the Shell Protein PduB

Journal Article · Mon May 16 00:00:00 EDT 2022 · Journal of Bacteriology

DOI:https://doi.org/10.1128/jb.00576-21· OSTI ID:1980758

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Northwestern Univ., Evanston, IL (United States); Northwestern Univ., Evanston, IL (United States)
Northwestern Univ., Evanston, IL (United States)

Bacterial microcompartments (MCPs) are protein-based organelles that house the enzymatic machinery for metabolism of niche carbon sources, allowing enteric pathogens to outcompete native microbiota during host colonization. While much progress has been made toward understanding MCP biogenesis, questions still remain regarding the mechanism by which core MCP enzymes are enveloped within the MCP protein shell. Here, we explore the hypothesis that the shell protein PduB is responsible for linking the shell of the 1,2-propanediol utilization (Pdu) MCP from Salmonella enterica serovar Typhimurium LT2 to its enzymatic core. Using fluorescent reporters, we demonstrate that all members of the Pdu enzymatic core are encapsulated in Pdu MCPs. We also demonstrate that PduB is critical for linking the entire Pdu enzyme core to the MCP shell. Using MCP purifications, transmission electron microscopy, and fluorescence microscopy, we find that shell assembly can be decoupled from the enzymatic core, as apparently empty MCPs are formed in Salmonella strains lacking PduB. Mutagenesis studies reveal that PduB is incorporated into the Pdu MCP shell via a conserved, lysine-mediated hydrogen bonding mechanism. Finally, growth assays and system-level pathway modeling reveal that unencapsulated pathway performance is strongly impacted by enzyme concentration, highlighting the importance of minimizing polar effects when conducting these functional assays. Together, these results provide insight into the mechanism of enzyme encapsulation within Pdu MCPs and demonstrate that the process of enzyme encapsulation and shell assembly are separate processes in this system, a finding that will aid future efforts to understand MCP biogenesis.

View Accepted Manuscript (DOE)

Research Organization:: Northwestern Univ., Evanston, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); US Army Research Office (ARO); USDOE Office of Science (SC)

Grant/Contract Number:: SC0019337

OSTI ID:: 1980758

Journal Information:: Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 9 Vol. 204; ISSN 0021-9193

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

1
2-propanediol utilization
59 BASIC BIOLOGICAL SCIENCES
Microbiology
Salmonella
bacterial microcompartment
enteric pathogens
metabolosome
organelle assembly
polar effect
shell protein

Linking the Salmonella enterica 1,2-Propanediol Utilization Bacterial Microcompartment Shell to the Enzymatic Core via the Shell Protein PduB

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