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Title: The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene

Journal Article · · Molecular Microbiology
DOI:https://doi.org/10.1111/mmi.13423· OSTI ID:1328044
 [1];  [2];  [2];  [2];  [1]
  1. Iowa State Univ., Ames, IA (United States)
  2. Univ. of California, Los Angeles, CA (United States)
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Bacterial microcompartments (MCPs) are complex organelles that consist of metabolic enzymes encapsulated within a protein shell. Here, we investigate the function of the PduJ MCP shell protein. PduJ is 80% identical in amino acid sequence to PduA and both are major shell proteins of the 1,2-propanediol (1,2-PD) utilization (Pdu) MCP of Salmonella. Prior studies showed that PduA mediates the transport of 1,2-PD (the substrate) into the Pdu MCP. Surprisingly, however, results presented here establish that PduJ has no role 1,2-PD transport. The crystal structure revealed that PduJ was nearly identical to that of PduA and, hence, offered no explanation for their differential functions. Interestingly, however, when a pduJ gene was placed at the pduA chromosomal locus, the PduJ protein acquired a new function, the ability to mediate 1,2-PD transport into the Pdu MCP. To our knowledge, these are the first studies to show that that gene location can determine the function of a MCP shell protein. We propose that gene location dictates protein-protein interactions essential to the function of the MCP shell.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Center for Research Resources; National Institute of General Medical Sciences (NIGMS)
Grant/Contract Number:
R01AI081146; T32-GM008496; 5P41RR015301-10; 8 P41 GM103403-10; AC02-06CH11357
OSTI ID:
1328044
Journal Information:
Molecular Microbiology, Vol. 101, Issue 5; ISSN 0950-382X
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
ENGLISH
Citation Metrics:
Cited by: 23 works
Citation information provided by
Web of Science

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Cited By (7)

Building a toolbox of protein scaffolds for future immobilization of biocatalysts journal July 2018
Functional protein shells fabricated from the self-assembling protein sheets of prokaryotic organelles journal January 2020
Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment of Salmonella journal December 2019
The N Terminus of the PduB Protein Binds the Protein Shell of the Pdu Microcompartment to Its Enzymatic Core journal January 2017
Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications journal July 2017
Broad-scale redistribution of mRNA abundance and transcriptional machinery in response to growth rate in Salmonella enterica serovar Typhimurium journal October 2017
Successive Paradigm Shifts in the Bacterial Cell Cycle and Related Subjects journal March 2019

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