Selective molecular transport through the protein shell of a bacterial microcompartment organelle

Chowdhury, Chiranjit; Chun, Sunny; Pang, Allan; Sawaya, Michael R.; Sinha, Sharmistha; Yeates, Todd O.; Bobik, Thomas A.

doi:10.1073/pnas.1423672112

Selective molecular transport through the protein shell of a bacterial microcompartment organelle

Journal Article · Mon Feb 23 00:00:00 EST 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1423672112· OSTI ID:1172992

Chowdhury, Chiranjit ^[1]; Chun, Sunny ^[2]; Pang, Allan ^[3]; Sawaya, Michael R. ^[3]; Sinha, Sharmistha ^[1]; Yeates, Todd O. ^[4]; Bobik, Thomas A. ^[1]

Iowa State Univ., Ames, IA (United States). Roy J. Carver Dept. of Biochemistry, Biophysics, and Molecular Biology
Univ. of California, Los Angeles, CA (United States). Molecular Biology Inst.
Univ. of California, Los Angeles, CA (United States). UCLA-DOE Inst. for Genomics and Proteomics
Univ. of California, Los Angeles, CA (United States). Molecular Biology Inst. UCLA-DOE Inst. for Genomics and Proteomics. Dept. of Chemistry and Biochemistry

Bacterial microcompartments are widespread prokaryotic organelles that have important and diverse roles ranging from carbon fixation to enteric pathogenesis. Current models for microcompartment function propose that their outer protein shell is selectively permeable to small molecules, but whether a protein shell can mediate selective permeability and how this occurs are unresolved questions. In this paper, biochemical and physiological studies of structure-guided mutants are used to show that the hexameric PduA shell protein of the 1,2-propanediol utilization (Pdu) microcompartment forms a selectively permeable pore tailored for the influx of 1,2-propanediol (the substrate of the Pdu microcompartment) while restricting the efflux of propionaldehyde, a toxic intermediate of 1,2-propanediol catabolism. Crystal structures of various PduA mutants provide a foundation for interpreting the observed biochemical and phenotypic data in terms of molecular diffusion across the shell. Finally and overall, these studies provide a basis for understanding a class of selectively permeable channels formed by nonmembrane proteins.

View Accepted Manuscript (DOE)

Research Organization:: Iowa State Univ., Ames, IA (United States); Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: National Inst. of Health (NIH) (United States); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1172992

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 10 Vol. 112; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
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Salmonella
carboxysome
microcompartment
protein channel

Selective molecular transport through the protein shell of a bacterial microcompartment organelle

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