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PioABC-Dependent Fe(II) Oxidation during Photoheterotrophic Growth on an Oxidized Carbon Substrate Increases Growth Yield

Journal Article · · Applied and Environmental Microbiology
DOI:https://doi.org/10.1128/aem.00974-22· OSTI ID:1980754
 [1];  [2];  [2];  [3];  [2];  [2];  [2]
  1. University of Minnesota, Saint Paul, MN (United States); Univ. of Minnesota, Minneapolis, MN (United States)
  2. University of Minnesota, Saint Paul, MN (United States)
  3. Univ. of Minnesota, Minneapolis, MN (United States)
+ Show Author Affiliations

Microorganisms that carry out Fe(II) oxidation play a major role in biogeochemical cycling of iron in environments with low oxygen. Fe(II) oxidation has been largely studied in the context of autotrophy. Here, we show that the anoxygenic phototroph, Rhodopseudomonas palustris CGA010, carries out Fe(II) oxidation during photoheterotrophic growth with an oxidized carbon source, malate, leading to an increase in cell yield and allowing more carbon to be directed to cell biomass. We probed the regulatory basis for this by transcriptome sequencing (RNA-seq) and found that the expression levels of the known pioABC Fe(II) oxidation genes in R. palustris depended on the redox-sensing two-component system, RegSR, and the oxidation state of the carbon source provided to cells. This provides the first mechanistic demonstration of mixotrophic growth involving reducing power generated from both Fe(II) oxidation and carbon assimilation.

Research Organization:
Univ. of Minnesota, Minneapolis, MN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); US Office of Naval Research (ONR)
Grant/Contract Number:
SC0020252
OSTI ID:
1980754
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 15 Vol. 88; ISSN 0099-2240
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Biotechnology & applied microbiology
Fe(II) oxidation
Rhodopseudomonas palustris
anoxygenic photosynthesis
microbiology