Genes essential for phototrophic growth by a purple alphaproteobacterium: Genes for phototrophic growth

Yang, Jianming; Yin, Liang; Lessner, Faith H.; Nakayasu, Ernesto S.; Payne, Samuel H.; Fixen, Kathryn R.; Gallagher, Larry; Harwood, Caroline S.

doi:10.1111/1462-2920.13852

Genes essential for phototrophic growth by a purple alphaproteobacterium: Genes for phototrophic growth

Journal Article · Mon Jul 24 00:00:00 EDT 2017 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.13852· OSTI ID:1406784

Yang, Jianming ^[1]; Yin, Liang ^[2]; Lessner, Faith H. ^[3]; Nakayasu, Ernesto S. ^[4]; Payne, Samuel H. ^[4]; Fixen, Kathryn R. ^[2]; Gallagher, Larry ^[5]; Harwood, Caroline S. ^[2]

Key Lab of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao Shandong Province People's Republic of China; Department of Microbiology, University of Washington, Seattle WA USA
Department of Microbiology, University of Washington, Seattle WA USA
Department of Biological Sciences, University of Arkansas, Fayetteville AR USA
Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA USA
Department of Genome Sciences, University of Washington, Seattle WA USA

Anoxygenic purple phototrophic bacteria have served as important models for studies of photophosphorylation. The pigment-protein complexes responsible for converting light energy to ATP are relatively simple and these bacteria can grow heterotrophically under aerobic conditions, thus allowing for the study of mutants defective in photophosphorylation. In the past, genes responsible for anoxygenic phototrophic growth have been identified in a number of different bacterial species. Here we systematically studied the genetic basis for this metabolism by using Tn-seq to identify genes essential for the anaerobic growth of the purple bacterium Rhodopseudomonas palustris on acetate in light. We identified 171 genes required for growth in this condition, 35 of which are annotated as photosynthesis genes. Among these are a few new genes not previously shown to be essential for phototrophic growth. We verified the essentiality of many of the genes we identified by analyzing the phenotypes of mutants we generated by Tn mutagenesis that had altered pigmentation. We used directed mutagenesis to verify that the R. palustris NADH:quinone oxidoreductase complex IE is essential for phototrophic growth. As a complement to the genetic data, we carried out proteomics experiments in which we found that 429 proteins were present in significantly higher amounts in cells grown anaerobically in light compared to aerobically. Among these were proteins encoded by subset of the phototrophic growth-essential genes.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1406784

Report Number(s):: PNNL-SA-124648; 48582; KP1601010

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 9 Vol. 19; ISSN 1462-2912

Publisher:: Wiley

Country of Publication:: United States

Language:: English

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