Genes essential for phototrophic growth by a purple alphaproteobacterium

Yang, Jianming; Yin, Liang; Lessner, Faith H.; Nakayasu, Ernesto S.; Payne, Samuel H.; Fixen, Kathryn R.; Gallagher, Larry; Harwood, Caroline S.

doi:10.1111/1462-2920.13852

Title: Genes essential for phototrophic growth by a purple alphaproteobacterium

Journal Article · Mon Jul 24 00:00:00 EDT 2017 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.13852· OSTI ID:1390349

Yang, Jianming ^[1]; Yin, Liang ^[2]; Lessner, Faith H. ^[3]; Nakayasu, Ernesto S. ^[4]; Payne, Samuel H. ^[4]; Fixen, Kathryn R. ^[2]; Gallagher, Larry ^[5]; Harwood, Caroline S. ^[2]

Key Lab of Applied Mycology, College of Life Sciences Qingdao Agricultural University Qingdao Shandong Province People's Republic of China, Department of Microbiology University of Washington Seattle WA USA
Department of Microbiology University of Washington Seattle WA USA
Department of Biological Sciences University of Arkansas Fayetteville AR USA
Biological Sciences Division Pacific Northwest National Laboratory Richland WA USA
Department of Genome Sciences University of Washington Seattle WA USA

Summary Tn‐seq was used to identify genes essential for phototrophic growth by the purple bacterium Rhodopseudomonas palustris . About 167 genes required for anaerobic growth on acetate in light were identified, 35 of which are annotated as photosynthesis genes. The essentiality of many of these genes by analysing the phenotypes of independently generated mutants that had altered pigmentation was verified. Three genes were identified, two possibly involved in biogenesis of the membrane‐bound photosynthetic apparatus and one for phosphatidylcholine biosynthesis, that were not known to be essential for phototrophic growth. Site‐directed mutagenesis was used to show that the NADH:quinone oxidoreductase complex I _E was essential for phototrophic growth under strictly anaerobic conditions and appeared to play a role in reverse electron transport to generate NADH. A homologous NADH:quinone oxidoreductase complex I _A likely operates in the opposite direction to oxidize NADH. The operation of the two enzymes in opposition would allow R. palustris to maintain redox balance. As a complement to the genetic data, proteomics experiments were carried out in which it was found that 408 proteins were present in significantly higher amounts in cells grown anaerobically in light compared with aerobically. Among these were proteins encoded by subset of the phototrophic growth‐essential genes.

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Sponsoring Organization:: USDOE

OSTI ID:: 1390349

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Vol. 19 Journal Issue: 9; ISSN 1462-2912

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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