A pectin methyltransferase modulates polysaccharide dynamics and interactions in Arabidopsis primary cell walls: Evidence from solid-state NMR

Kirui, Alex; Du, Juan; Zhao, Wancheng; Barnes, William; Kang, Xue; Anderson, Charles T.; Xiao, Chaowen; Wang, Tuo

doi:10.1016/j.carbpol.2021.118370

A pectin methyltransferase modulates polysaccharide dynamics and interactions in Arabidopsis primary cell walls: Evidence from solid-state NMR

Journal Article · Thu Jun 24 00:00:00 EDT 2021 · Carbohydrate Polymers

DOI:https://doi.org/10.1016/j.carbpol.2021.118370· OSTI ID:1976908

Kirui, Alex ^[1]; Du, Juan ^[2]; Zhao, Wancheng ^[3]; Barnes, William ^[4]; Kang, Xue ^[5]; Anderson, Charles T. ^[6]; Xiao, Chaowen ^[2]; Wang, Tuo ^[3]

Louisiana State University, Baton Rouge, LA (United States); OSTI
Sichuan University, Chengdu (China)
Louisiana State University, Baton Rouge, LA (United States)
Pennsylvania State University, University Park, PA (United States); University of Georgia, Athens, GA (United States)
Louisiana State University, Baton Rouge, LA (United States); Ningbo University, Zhejiang (China)
Pennsylvania State University, University Park, PA (United States)

Plant cell walls contain cellulose embedded in matrix polysaccharides. Understanding carbohydrate structures and interactions is critical to the production of biofuel and biomaterials using these natural resources. Here we present a solid-state NMR study of cellulose and pectin in ¹³C-labeled cell walls of Arabidopsis wild-type and mutant plants. Using 1D ¹³C and 2D ¹³C–¹³C correlation experiments, we detected a highly branched arabinan structure in qua2 and tsd2 samples, two allelic mutants for a pectin methyltransferase. Both mutants show close physical association between cellulose and the backbones of pectic homogalacturonan and rhamnogalacturonan-I. Relaxation and dipolar order parameters revealed enhanced microsecond dynamics due to polymer disorder in the mutants, but restricted motional amplitudes due to tighter pectin-cellulose associations. These molecular data shed light on polymer structure and packing in these two pectin mutants, helping to elucidate how pectin could influence cell wall architecture at the nanoscale, cell wall mechanics, and plant growth.

View Accepted Manuscript (DOE)

Research Organization:: Pennsylvania State University, University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1976908

Alternate ID(s):: OSTI ID: 1804567

Journal Information:: Carbohydrate Polymers, Journal Name: Carbohydrate Polymers Journal Issue: C Vol. 270; ISSN 0144-8617

Publisher:: Applied Science DirectCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Cellulose
Chemistry
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Plant cell wall
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Solid-state NMR

A pectin methyltransferase modulates polysaccharide dynamics and interactions in Arabidopsis primary cell walls: Evidence from solid-state NMR

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