Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis

Bing, Ryan G.; Straub, Christopher T.; Sulis, Daniel B.; Wang, Jack P.; Adams, Michael W.W.; Kelly, Robert M.

doi:10.1016/j.biortech.2022.126780

Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis

Journal Article · Sat Jan 29 00:00:00 EST 2022 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2022.126780· OSTI ID:1976891

Bing, Ryan G. ^[1]; Straub, Christopher T. ^[2]; Sulis, Daniel B. ^[2]; Wang, Jack P. ^[2]; Adams, Michael W.W. ^[3]; Kelly, Robert M. ^[2]

North Carolina State University, Raleigh, NC (United States); OSTI
North Carolina State University, Raleigh, NC (United States)
University of Georgia, Athens, GA (United States)

A variety of chemical and biological processes have been proposed for conversion of sustainable low-cost feedstocks into industrial products. Here, a biorefinery concept is formulated, modeled, and analyzed in which a naturally (hemi)cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii, is metabolically engineered to convert the carbohydrate content of lignocellulosic biomasses (i.e., soybean hulls, transgenic poplar) into green hydrogen and acetone. Experimental validation of C. bescii fermentative performance demonstrated 82% carbohydrate solubilization of soybean hulls and 55% for transgenic poplar. A detailed technical design, including equipment specifications, provides the basis for an economic analysis that establishes metabolic engineering targets. This robust industrial process leveraging metabolically engineered C. bescii yields 206 kg acetone and 25 kg H₂ per metric ton of soybean hull, or 174 kg acetone and 21 kg H₂ per metric ton transgenic poplar. Beyond this specific case, the model demonstrates industrial feasibility and economic advantages of thermophilic fermentation.

View Accepted Manuscript (DOE)

Research Organization:: University of Georgia, Athens, GA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); US Department of Agriculture (USDA); US DoEd GAANN Fellowship; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019391; SC0022192

OSTI ID:: 1976891

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 348; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (35)

Techno-economic analysis of biodiesel and ethanol co-production from lipid-producing sugarcane: Biodiesel and Ethanol Co-Production from Lipid-Producing Sugarcane Huang, Haibo; Long, Stephen; Singh, Vijay Biofuels, Bioproducts and Biorefining, Vol. 10, Issue 3 https://doi.org/10.1002/bbb.1640	journal	March 2016
Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity Straub, Christopher T.; Khatibi, Piyum A.; Otten, Jonathan K. Biotechnology and Bioengineering, Vol. 116, Issue 8 https://doi.org/10.1002/bit.26993	journal	May 2019
Metabolically engineered Caldicellulosiruptor bescii as a platform for producing acetone and hydrogen from lignocellulose Straub, Christopher T.; Bing, Ryan G.; Otten, Jonathan K. Biotechnology and Bioengineering, Vol. 117, Issue 12 https://doi.org/10.1002/bit.27529	journal	August 2020
Influence of Feedstock Particle Size on Lignocellulose Conversion—A Review Vidal, Bernardo C.; Dien, Bruce S.; Ting, K. C. Applied Biochemistry and Biotechnology, Vol. 164, Issue 8 https://doi.org/10.1007/s12010-011-9221-3	journal	March 2011
Basics and industrial applications of pressure swing adsorption (PSA), the modern way to separate gas Wiessner, Frank G. Gas Separation & Purification, Vol. 2, Issue 3 https://doi.org/10.1016/0950-4214(88)80026-4	journal	September 1988
A demonstration study of ethanol production from sweet sorghum stems with advanced solid state fermentation technology Li, Shizhong; Li, Guangming; Zhang, Lei Applied Energy, Vol. 102 https://doi.org/10.1016/j.apenergy.2012.09.060	journal	February 2013
Impact of the biorefinery size on the logistics of corn stover supply – A scenario analysis Wang, Yu; Ebadian, Mahmood; Sokhansanj, Shahab Applied Energy, Vol. 198 https://doi.org/10.1016/j.apenergy.2017.03.056	journal	July 2017
High concentration ethanol production from corncob residues by fed-batch strategy Liu, Kai; Lin, Xiaohui; Yue, Jun Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.11.013	journal	July 2010
Degradation of high loads of crystalline cellulose and of unpretreated plant biomass by the thermophilic bacterium Caldicellulosiruptor bescii Basen, Mirko; Rhaesa, Amanda M.; Kataeva, Irina Bioresource Technology, Vol. 152 https://doi.org/10.1016/j.biortech.2013.11.024	journal	January 2014
Recent advances in commercial biorefineries for lignocellulosic ethanol production: Current status, challenges and future perspectives Raj, Tirath; Chandrasekhar, K.; Naresh Kumar, A. Bioresource Technology, Vol. 344 https://doi.org/10.1016/j.biortech.2021.126292	journal	January 2022
The progress in water gas shift and steam reforming hydrogen production technologies – A review LeValley, Trevor L.; Richard, Anthony R.; Fan, Maohong International Journal of Hydrogen Energy, Vol. 39, Issue 30 https://doi.org/10.1016/j.ijhydene.2014.08.041	journal	October 2014
Greenhouse gas assessment of soybean production: implications of land use change and different cultivation systems Castanheira, Érica Geraldes; Freire, Fausto Journal of Cleaner Production, Vol. 54 https://doi.org/10.1016/j.jclepro.2013.05.026	journal	September 2013
Engineering redox-balanced ethanol production in the cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii Williams-Rhaesa, Amanda M.; Rubinstein, Gabriel M.; Scott, Israel M. Metabolic Engineering Communications, Vol. 7 https://doi.org/10.1016/j.mec.2018.e00073	journal	December 2018
Impact of feedstock quality and variation on biochemical and thermochemical conversion Li, Chenlin; Aston, John E.; Lacey, Jeffrey A. Renewable and Sustainable Energy Reviews, Vol. 65 https://doi.org/10.1016/j.rser.2016.06.063	journal	November 2016
Lignocellulosic biorefineries: The current state of challenges and strategies for efficient commercialization Usmani, Zeba; Sharma, Minaxi; Awasthi, Abhishek Kumar Renewable and Sustainable Energy Reviews, Vol. 148 https://doi.org/10.1016/j.rser.2021.111258	journal	September 2021
Ordered mesoporous silica particles and Si-MCM-41 for the adsorption of acetone: A comparative study Hung, Chinte; Bai, Hsunling; Karthik, Mani Separation and Purification Technology, Vol. 64, Issue 3 https://doi.org/10.1016/j.seppur.2008.10.020	journal	January 2009
The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology Sandberg, Troy E.; Salazar, Michael J.; Weng, Liam L. Metabolic Engineering, Vol. 56 https://doi.org/10.1016/j.ymben.2019.08.004	journal	December 2019
Compositional Analysis of Defatted Syrup from a Corn Ethanol Dry-Grind Process as a Feedstock for Biobased Products Adom, Felix; Fan, Jiqing; Davis, Jamie ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 5 https://doi.org/10.1021/sc400508p	journal	April 2014
Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii Straub, Christopher T.; Khatibi, Piyum A.; Wang, Jack P. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-11376-6	journal	August 2019
Development of a thermophilic coculture for corn fiber conversion to ethanol Beri, Dhananjay; York, William S.; Lynd, Lee R. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15704-z	journal	April 2020
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield Shaw, A. J.; Podkaminer, K. K.; Desai, S. G. Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774 https://doi.org/10.1073/pnas.0801266105	journal	September 2008
Genetic Transformation of Populus trichocarpa Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants Song, Jingyuan; Lu, Shanfa; Chen, Zenn-Zong Plant and Cell Physiology, Vol. 47, Issue 11, p. 1582-1589 https://doi.org/10.1093/pcp/pcl018	journal	November 2006
The Economics of Harvesting Corn Cobs for Energy Erickson, Matthew J.; Dobbins, Craig; Tyner, Wallace E. Crop Management, Vol. 10, Issue 1 https://doi.org/10.1094/CM-2011-0324-02-RS	journal	January 2011
Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose Bing, Ryan G.; Sulis, Daniel B.; Wang, Jack P. Environmental Microbiology Reports, Vol. 13, Issue 3 https://doi.org/10.1111/1758-2229.12943	journal	March 2021
A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii Lipscomb, Gina L.; Conway, Jonathan M.; Blumer-Schuette, Sara E. Applied and Environmental Microbiology, Vol. 82, Issue 14 https://doi.org/10.1128/AEM.00570-16	journal	May 2016
Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii Zurawski, Jeffrey V.; Khatibi, Piyum A.; Akinosho, Hannah O. Applied and Environmental Microbiology, Vol. 83, Issue 17 https://doi.org/10.1128/AEM.00969-17	journal	June 2017
Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization Zurawski, Jeffrey V.; Conway, Jonathan M.; Lee, Laura L. Applied and Environmental Microbiology, Vol. 81, Issue 20 https://doi.org/10.1128/AEM.01622-15	journal	August 2015
Carbohydrate Utilization Patterns for the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Reveal Broad Growth Substrate Preferences VanFossen, A. L.; Verhaart, M. R. A.; Kengen, S. M. W. Applied and Environmental Microbiology, Vol. 75, Issue 24 https://doi.org/10.1128/AEM.01959-09	journal	October 2009
The cumene process for phenol-acetone production Zakoshansky, V. M. Petroleum Chemistry, Vol. 47, Issue 4 https://doi.org/10.1134/S096554410704007X	journal	July 2007
Reassessment of hydrogen tolerance in Caldicellulosiruptor saccharolyticus Willquist, Karin; Pawar, Sudhanshu S.; Van Niel, Ed WJ Microbial Cell Factories, Vol. 10, Issue 1 https://doi.org/10.1186/1475-2859-10-111	journal	January 2011
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0155-1	journal	October 2014
Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood Herring, Christopher D.; Kenealy, William R.; Joe Shaw, A. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0536-8	journal	June 2016
Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar Straub, Christopher T.; Bing, Ryan G.; Wang, Jack P. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01675-2	journal	March 2020
Methylation by a Unique α-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725 Chung, Daehwan; Farkas, Joel; Huddleston, Jennifer R. PLoS ONE, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pone.0043844	journal	August 2012
Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover Humbird, D.; Davis, R.; Tao, L. https://doi.org/10.2172/1013269	report	March 2011

Similar Records

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

Journal Article · Tue Aug 06 20:00:00 EDT 2019 · Nature Communications · OSTI ID:1613208

Metabolically engineered Caldicellulosiruptor bescii as a platform for producing acetone and hydrogen from lignocellulose

Journal Article · Sun Aug 23 20:00:00 EDT 2020 · Biotechnology and Bioengineering · OSTI ID:1786038

Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity

Journal Article · Mon May 20 20:00:00 EDT 2019 · Biotechnology and Bioengineering · OSTI ID:1515088

Related Subjects

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
59 BASIC BIOLOGICAL SCIENCES
Caldicellulosiruptor bescii
acetone
extreme thermophiles
green hydrogen
technoeconomic analysis

Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis

Citation Formats

References (35)

Similar Records

Related Subjects