Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

Straub, Christopher T.; Khatibi, Piyum A.; Wang, Jack P.; Conway, Jonathan M.; Williams-Rhaesa, Amanda M.; Peszlen, Ilona M.; Chiang, Vincent L.; Adams, Michael W.  W.; Kelly, Robert M.

doi:10.1038/s41467-019-11376-6

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

Journal Article · Wed Aug 07 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-11376-6· OSTI ID:1613208

Straub, Christopher T. ^[1]; ^[2]; Wang, Jack P. ^[2]; ^[2]; ^[3]; ^[2]; Chiang, Vincent L. ^[2]; Adams, Michael W. W. ^[3]; ^[2]

North Carolina State Univ., Raleigh, NC (United States); DOE/OSTI
North Carolina State Univ., Raleigh, NC (United States)
Univ. of Georgia, Athens, GA (United States)

Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0019391

OSTI ID:: 1613208

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 10; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar Straub, Christopher T.; Bing, Ryan G.; Wang, Jack P. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01675-2	journal	March 2020

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