Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid

Zhang, Pingbo; Kirby, David; Dufresne, Craig; Chen, Yan; Turner, Randi; Ferri, Sara; Edward, Deepak P.; Van Eyk, Jennifer E.; Semba, Richard D.

doi:10.1002/pmic.201500188

Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid

Journal Article · Tue Feb 02 00:00:00 EST 2016 · Proteomics

DOI:https://doi.org/10.1002/pmic.201500188· OSTI ID:1975933

Zhang, Pingbo ^[1]; Kirby, David ^[1]; Dufresne, Craig ^[2]; Chen, Yan ^[1]; Turner, Randi ^[1]; Ferri, Sara ^[1]; Edward, Deepak P. ^[3]; Van Eyk, Jennifer E. ^[4]; Semba, Richard D. ^[1]

Johns Hopkins Univ., Baltimore, MD (United States). School of Medicine
Thermo Fisher Scientific, West Palm Beach, FL (United States)
Johns Hopkins Univ., Baltimore, MD (United States). School of Medicine; King Khaled Eye Specialist Hospital (Saudi Arabia)
Cedars-Sinai Medical Center, Los Angeles, CA (United States)

The iris is a fine structure that controls the amount of light that enters the eye. The ciliary body controls the shape of the lens and produces aqueous humor. The retinal pigment epithelium and choroid (RPE/choroid) are essential in supporting the retina and absorbing light energy that enters the eye. Here, proteins were extracted from iris, ciliary body, and RPE/choroid tissues of eyes from five individuals and fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. In iris, ciliary body, and RPE/choroid, we identified 2959, 2867, and 2755 nonredundant proteins with peptide and protein false-positive rates of <0.1% and <1%, respectively. Forty-three unambiguous protein isoforms were identified in iris, ciliary body, and RPE/choroid. Four “missing proteins” were identified in ciliary body based on ≥2 proteotypic peptides. The mass spectrometric proteome database of the human iris, ciliary body, and RPE/choroid may serve as a valuable resource for future investigations of the eye in health and disease. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD001424 and PXD002194.

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1975933

Journal Information:: Proteomics, Journal Name: Proteomics Journal Issue: 7 Vol. 16; ISSN 1615-9853

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
biomedicine
choroid
ciliary body
eye
iris
retinal pigment epithelium

Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid

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References (12)

Cited By (4)

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