Laboratory preparation of DNPH derivatives of carbonyl compounds on Sep-Pak{reg_sign} cartridges for quality assurance purposes
- ManTech Environmental Technology Inc., Research Triangle Park, NC (United States)
Aldehydes and ketones are receiving increased attention both as hazardous substances and as promoters in the photochemical formation of ozone in the atmosphere. They enter the atmosphere in the exhaust of motor vehicles and other equipment using hydrocarbon and alcohol fuels. Formaldehyde, the most prevalent aldehyde, is widely used as a preservative, a textile-treatment agent, and an intermediate in the manufacture of urea-formaldehyde and phenol-formaldehyde resins. The formaldehyde concentration ranges for several types of environments are presented. Waters Sep-Pak{reg_sign} DNPH-Silica cartridges are convenient, reproducible sampling devices for quantifying aldehydes and ketones in gases, including air. These cartridges trap the compounds by reacting them with the DNPH, 2,4-Dinitrophenylhydrazine, on the cartridge to form stable hydrazone derivatives. Derivatives are later eluted and analyzed by HPLC. Cartridges spiked in the laboratory are used for quality assurance and instrument performance verification.
- OSTI ID:
- 197519
- Report Number(s):
- CONF-9405167--
- Country of Publication:
- United States
- Language:
- English
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