Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix-assisted laser desorption–ionization mass spectrometry

Schenkel, T.; Snijders, A. M.; Nakamura, K.; Seidl, P. A.; Mak, B.; Obst-Huebl, L.; Knobel, H.; Pong, I.; Persaud, A.; van Tilborg, J.; Ostermayr, T.; Steinke, S.; Blakely, E. A.; Ji, Q.; Javey, A.; Kapadia, R.; Geddes, C. R.; Esarey, E.

doi:10.1063/5.0128667

Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix-assisted laser desorption–ionization mass spectrometry

Journal Article · Mon Jan 30 00:00:00 EST 2023 · Applied Physics Letters

DOI:https://doi.org/10.1063/5.0128667· OSTI ID:1971344

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Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Eurofins Materials Science Netherlands BV, Eindhoven, (Netherlands)
University of California, Berkeley, CA (United States)

Nanostructured surfaces enhance ion yields in matrix-assisted laser desorption–ionization mass spectrometry (MALDI-MS). The spike protein complex, S1, is one fingerprint signature of Sars-CoV-2 with a mass of 75 kDa. Here, we show that MALDI-MS yields of Sars-CoV-2 spike protein ions in the 100 kDa range are enhanced 50-fold when the matrix–analyte solution is placed on substrates that are coated with a dense forest of multi-walled carbon nanotubes, compared to yields from uncoated substrates. Nanostructured substrates can support the development of mass spectrometry techniques for sensitive pathogen detection and environmental monitoring.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC), High Energy Physics (HEP)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1971344

Journal Information:: Applied Physics Letters, Journal Name: Applied Physics Letters Journal Issue: 5 Vol. 122; ISSN 0003-6951

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix-assisted laser desorption–ionization mass spectrometry

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