Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2

Journal Article · · Glycobiology (Online)
 [1];  [2];  [2];  [2]
  1. Univ. of Georgia, Athens, GA (United States); Complex Carbohydrate Research Center, University of Georgia
  2. Univ. of Georgia, Athens, GA (United States)
+ Show Author Affiliations

The current emergence of the novel coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands the development of new therapeutic strategies to prevent rapid progress of mortalities. The coronavirus spike (S) protein, which facilitates viral attachment, entry and membrane fusion is heavily glycosylated and plays a critical role in the elicitation of the host immune response. The spike protein is comprised of two protein subunits (S1 and S2), which together possess 22 potential N-glycosylation sites. Herein, we report the glycosylation mapping on spike protein subunits S1 and S2 expressed on human cells through high-resolution mass spectrometry. We have characterized the quantitative N-glycosylation profile on spike protein and interestingly, observed unexpected O-glycosylation modifications on the receptor-binding domain of spike protein subunit S1. Even though O-glycosylation has been predicted on the spike protein of SARS-CoV-2, this is the first report of experimental data for both the site of O-glycosylation and identity of the O-glycans attached on the subunit S1. Our data on the N- and O-glycosylation are strengthened by extensive manual interpretation of each glycopeptide spectra in addition to using bioinformatics tools to confirm the complexity of glycosylation in the spike protein. Here, the elucidation of the glycan repertoire on the spike protein provides insights into the viral binding studies and more importantly, propels research toward the development of a suitable vaccine candidate.

Research Organization:
Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); US National Institutes of Health
Grant/Contract Number:
SC0015662
OSTI ID:
1964028
Alternate ID(s):
OSTI ID: 1852016
Journal Information:
Glycobiology (Online), Journal Name: Glycobiology (Online) Journal Issue: 12 Vol. 30; ISSN 1460-2423
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

References (26)

Exploitation of glycosylation in enveloped virus pathobiology journal October 2019
Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion journal December 2020
Database Analysis of O-Glycosylation Sites in Proteins journal February 2001
Adaptive immune activation: glycosylation does matter journal November 2013
A new coronavirus associated with human respiratory disease in China journal February 2020
Structural basis for human coronavirus attachment to sialic acid receptors journal June 2019
Why Glycosylation Matters in Building a Better Flu Vaccine journal October 2019
Glycomic and glycoproteomic analysis of glycoproteins—a tutorial journal June 2017
Database Analysis of O-Glycosylation Sites in Proteins journal February 2001
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China journal February 2020
Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion journal February 2019
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor journal April 2020
Structural Constraints Determine the Glycosylation of HIV-1 Envelope Trimers journal June 2015
Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture journal January 2018
Therapeutic options for the 2019 novel coronavirus (2019-nCoV) journal February 2020
Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology journal April 2013
A pneumonia outbreak associated with a new coronavirus of probable bat origin journal February 2020
Structural basis of receptor recognition by SARS-CoV-2 journal March 2020
The proximal origin of SARS-CoV-2 journal March 2020
Why Glycosylation Matters in Building a Better Flu Vaccine journal October 2019
Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST journal March 2013
Symbol Nomenclature for Graphical Representations of Glycans journal November 2015
Global aspects of viral glycosylation journal March 2018
Emerging viruses and current strategies for vaccine intervention journal April 2019
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation journal February 2020
Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2 journal March 2020

Cited By (3)

L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus journal July 2021
Can SARS-CoV-2 Virus Use Multiple Receptors to Enter Host Cells? journal January 2021
Does COVID-19 Vaccination Warrant the Classical Principle " ofelein i mi vlaptin"? text January 2021

Figures / Tables (6)

Figure 1(p. 16)figure Figure 1
Figure 2(p. 17)figure Figure 2
Figure 3(p. 17)figure Figure 3
Figure 4(p. 19)figure Figure 4
Figure 5(p. 19)figure Figure 5
Figure 6(p. 20)figure Figure 6

Similar Records

Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2
Journal Article · Mon May 04 00:00:00 EDT 2020 · Glycobiology (Online) · OSTI ID:1852016
Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2
Journal Article · Thu Oct 29 00:00:00 EDT 2020 · Glycobiology (Online) · OSTI ID:1852017
Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2
Journal Article · Thu Oct 29 00:00:00 EDT 2020 · Glycobiology (Online) · OSTI ID:1963813

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COVID-19
S1 S2 glycosylation
SARS-CoV-2 glycosylation
coronavirus vaccine
spike protein