Uncovering a miltiradiene biosynthetic gene cluster in the Lamiaceae reveals a dynamic evolutionary trajectory

Bryson, Abigail E.; Lanier, Emily R.; Lau, Kin H.; Hamilton, John P.; Vaillancourt, Brieanne; Mathieu, Davis; Yocca, Alan E.; Miller, Garret P.; Edger, Patrick P.; Buell, C. Robin; Hamberger, Björn

doi:10.1038/s41467-023-35845-1

Uncovering a miltiradiene biosynthetic gene cluster in the Lamiaceae reveals a dynamic evolutionary trajectory

Journal Article · Fri Jan 20 00:00:00 EST 2023 · Nature Communications

DOI:https://doi.org/10.1038/s41467-023-35845-1· OSTI ID:1970237

^[1]; ^[2]; Lau, Kin H. ^[3]; Hamilton, John P. ^[4]; ^[4]; Mathieu, Davis ^[2]; ^[2]; ^[2]; Edger, Patrick P. ^[2]; Buell, C. Robin ^[2]; ^[2]

Michigan State Univ., East Lansing, MI (United States); Michigan State University Department of Biochemistry
Michigan State Univ., East Lansing, MI (United States)
Michigan State Univ., East Lansing, MI (United States); Van Andel Inst., Grand Rapids, MI (United States)
Michigan State Univ., East Lansing, MI (United States); Univ. of Georgia, Athens, GA (United States)

The spatial organization of genes within plant genomes can drive evolution of specialized metabolic pathways. Terpenoids are important specialized metabolites in plants with diverse adaptive functions that enable environmental interactions. Here, we report the genome assemblies of Prunella vulgaris, Plectranthus barbatus, and Leonotis leonurus. We investigate the origin and subsequent evolution of a diterpenoid biosynthetic gene cluster (BGC) together with other seven species within the Lamiaceae (mint) family. Based on core genes found in the BGCs of all species examined across the Lamiaceae, we predict a simplified version of this cluster evolved in an early Lamiaceae ancestor. The current composition of the extant BGCs highlights the dynamic nature of its evolution. We elucidate the terpene backbones generated by the Callicarpa americana BGC enzymes, including miltiradiene and the terpene (+)-kaurene, and show oxidization activities of BGC cytochrome P450s. Our work reveals the fluid nature of BGC assembly and the importance of genome structure in contributing to the origin of metabolites.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States)

Sponsoring Organization:: NSF-IMPACTS; National Science Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Georgia Advanced Computing Resource Center

Grant/Contract Number:: SC0018409

OSTI ID:: 1970237

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 14; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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