Generation of a chromosome-scale genome assembly of the insect-repellent terpenoid-producing Lamiaceae species, Callicarpa americana

Hamilton, John P.; Godden, Grant T.; Lanier, Emily; Bhat, Wajid Waheed; Kinser, Taliesin J.; Vaillancourt, Brieanne; Wang, Haiyan; Wood, Joshua C.; Jiang, Jiming; Soltis, Pamela S.; Soltis, Douglas E.; Hamberger, Bjoern; Buell, C. Robin

doi:10.1093/gigascience/giaa093

Generation of a chromosome-scale genome assembly of the insect-repellent terpenoid-producing Lamiaceae species, Callicarpa americana

Journal Article · Mon Sep 07 00:00:00 EDT 2020 · GigaScience

DOI:https://doi.org/10.1093/gigascience/giaa093· OSTI ID:1764716

^[1]; ^[2]; ^[3]; ^[3]; ^[2]; ^[3]; Wang, Haiyan ^[3]; ^[3]; ^[3]; ^[2]; ^[2]; ^[3]; ^[3]

Michigan State Univ., East Lansing, MI (United States); Michigan State University Department of Plant Biology
Univ. of Florida, Gainesville, FL (United States)
Michigan State Univ., East Lansing, MI (United States)

Plants exhibit wide chemical diversity due to the production of specialized metabolites that function as pollinator attractants, defensive compounds, and signaling molecules. Lamiaceae (mints) are known for their chemodiversity and have been cultivated for use as culinary herbs, as well as sources of insect repellents, health-promoting compounds, and fragrance. We report the chromosome-scale genome assembly of Callicarpa americana L. (American beautyberry), a species within the early-diverging Callicarpoideae clade of Lamiaceae, known for its metallic purple fruits and use as an insect repellent due to its production of terpenoids. Using long-read sequencing and Hi-C scaffolding, we generated a 506.1-Mb assembly spanning 17 pseudomolecules with N50 contig and N50 scaffold sizes of 7.5 and 29.0 Mb, respectively. In all, 32,164 genes were annotated, including 53 candidate terpene synthases and 47 putative clusters of specialized metabolite biosynthetic pathways. Our analyses revealed 3 putative whole-genome duplication events, which, together with local tandem duplications, contributed to gene family expansion of terpene synthases. Kolavenyl diphosphate is a gateway to many of the bioactive terpenoids in C. americana; experimental validation confirmed that CamTPS2 encodes kolavenyl diphosphate synthase. Syntenic analyses with Tectona grandis L. f. (teak), a member of the Tectonoideae clade of Lamiaceae known for exceptionally strong wood resistant to insects, revealed 963 collinear blocks and 21,297 C. americana syntelogs. Access to the C. americana genome provides a road map for rapid discovery of genes encoding plant-derived agrichemicals and a key resource for understanding the evolution of chemical diversity in Lamiaceae.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494; SC0018409

OSTI ID:: 1764716

Journal Information:: GigaScience, Journal Name: GigaScience Journal Issue: 9 Vol. 9; ISSN 2047-217X

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Generation of a chromosome-scale genome assembly of the insect-repellant terpenoid-producing Lamiaceae species, Callicarpa americana Hamilton, John P.; Godden, Grant; Lanier, Emily Dryad https://doi.org/10.5061/dryad.931zcrjgj	dataset	January 2020
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